SPAG4 Antibody (H-6): sc-393115
- SPAG4 Antibody (H-6) is a mouse monoclonal IgG1 κ SPAG4 antibody provided at 200 µg/ml
- raised against amino acids 212-266 mapping within an internal region of SPAG4 of human origin
- SPAG4 Antibody (H-6) is recommended for detection of SPAG4 of human origin by WB, IP, IF and ELISA
- Anti-SPAG4 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPAG4 Antibody (H-6) for WB applications. These reagents are now offered in bundles with SPAG4 Antibody (H-6) (see ordering information below).
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SPAG4 Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPAG4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPAG4 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPAG4, or sperm associated antigen 4, is a 427 amino acid multi-pass membrane protein that plays a crucial role in the organization and assembly of outer dense fibers (ODFs) within the mammalian sperm flagellum. SPAG4 localization is particularly important as SPAG4 is found in the transient manchette and axoneme of elongating spermatids and epididymal sperm, where SPAG4 contributes to the structural integrity and functionality of the sperm tail. SPAG4 exists as a homodimer and interacts with Odf1, suggesting a significant role in the assembly of the cytoskeletal structures that are essential for sperm motility. Additionally, the presence of a SUN domain in SPAG4 indicates SPAG4′s potential involvement in nuclear-cytoskeletal connections, making SPAG4 a candidate for further investigation as a cancer marker.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPAG4 Antibody (H-6) References:
- Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme. | Shao, X., et al. 1999. Dev Biol. 211: 109-23. PMID: 10373309
- Spermatid manchette: plugging proteins to zero into the sperm tail. | Kierszenbaum, AL. 2001. Mol Reprod Dev. 59: 347-9. PMID: 11468770
- Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1. | Shao, X., et al. 2001. Mol Reprod Dev. 59: 410-6. PMID: 11468777
- Novel RING finger protein OIP1 binds to conserved amino acid repeats in sperm tail protein ODF1. | Zarsky, HA., et al. 2003. Biol Reprod. 68: 543-52. PMID: 12533418
- Human sperm associated antigen 4 (SPAG4) is a potential cancer marker. | Kennedy, C., et al. 2004. Cell Tissue Res. 315: 279-83. PMID: 14614621
- Identification of a novel gene SRG4 expressed at specific stages of mouse spermatogenesis. | Xing, XW., et al. 2004. Acta Biochim Biophys Sin (Shanghai). 36: 351-9. PMID: 15156277
- Rat Spag5 associates in somatic cells with endoplasmic reticulum and microtubules but in spermatozoa with outer dense fibers. | Fitzgerald, CJ., et al. 2006. Mol Reprod Dev. 73: 92-100. PMID: 16211599
- Nuclear envelope localization of human UNC84A does not require nuclear lamins. | Hasan, S., et al. 2006. FEBS Lett. 580: 1263-8. PMID: 16445915
- A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2. | Tarnasky, H., et al. 1998. Cytogenet Cell Genet. 81: 65-7. PMID: 9691178
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPAG4 Antibody (H-6) | sc-393115 | 200 µg/ml | $322.00 | |||
SPAG4 Antibody (H-6): m-IgG Fc BP-HRP Bundle | sc-530359 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPAG4 Antibody (H-6): m-IgGκ BP-HRP Bundle | sc-523810 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPAG4 Antibody (H-6): m-IgG1 BP-HRP Bundle | sc-544011 | 200 µg Ab; 20 µg BP | $361.00 | |||
SPAG4 Antibody (H-6) AC | sc-393115 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPAG4 Antibody (H-6) HRP | sc-393115 HRP | 200 µg/ml | $322.00 | |||
SPAG4 Antibody (H-6) FITC | sc-393115 FITC | 200 µg/ml | $336.00 | |||
SPAG4 Antibody (H-6) PE | sc-393115 PE | 200 µg/ml | $349.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 488 | sc-393115 AF488 | 200 µg/ml | $364.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 546 | sc-393115 AF546 | 200 µg/ml | $364.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 594 | sc-393115 AF594 | 200 µg/ml | $364.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 647 | sc-393115 AF647 | 200 µg/ml | $364.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 680 | sc-393115 AF680 | 200 µg/ml | $364.00 | |||
SPAG4 Antibody (H-6) Alexa Fluor® 790 | sc-393115 AF790 | 200 µg/ml | $364.00 |