Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SPA-1 CRISPR/Cas9 KO Plasmid (h): sc-406755
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPA-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPA-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPA-1 Antibody (B-7): sc-166219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPA-1 CRISPR/Cas9 KO Plasmid (h) | sc-406755 | 20 µg | $397.00 |
Overview
SIPA1 encodes SPA-1, a Rap1 GTPase-activating protein that accelerates GTP hydrolysis to limit Rap1 signaling and tune integrin-mediated adhesion, cytoskeletal dynamics, and cell migration. By modulating Rap1-dependent inside-out signaling, SPA-1 influences immune cell trafficking, cell–cell junction stability, and responses to extracellular cues. Altered SIPA1 expression or activity has been linked to dysregulated proliferation and motility programs relevant to tumor progression and metastatic potential. This biology makes SIPA1 a useful node for studying small GTPase networks, adhesion-dependent signaling, and microenvironment-driven cellular behaviors.
SPA-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SIPA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SIPA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SIPA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPA-1 protein expression.
This CRISPR knockout system enables efficient generation of SIPA1-deficient cell models for investigation of SPA-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SIPA1 exon(s) critical for SPA-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SIPA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPA-1 CRISPR/Cas9 KO Plasmid (h) and SPA-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SIPA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPA-1 HDR Plasmid (h) and SPA-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SIPA1 homology arms to support homology-directed repair at defined SIPA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.