SPA-1 Antibody (B-7): sc-166219

SPA-1 Antibody (B-7) is a mouse monoclonal IgM kappa light chain antibody that detects SPA-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPA-1 (B-7) antibody is available as the non-conjugated form. SPA-1 (signal-induced proliferation-associated gene-1) protein serves as a crucial regulator in hematopoietic progenitors and peripheral T cells, functioning primarily as a RAP1 GTPase-activating protein. SPA-1 protein is predominantly expressed in fetal and adult lymphohematopoietic tissues, and SPA-1 expression is significantly upregulated in response to various mitogenic stimuli in normal lymphocytes. SPA-1 protein′s ability to disrupt LFA-1-ICAM1-mediated adhesive interactions is vital for modulating T cell receptor signaling and subsequent interleukin-2 production, potentially through RAP1 activity inhibition. SPA-1 protein also interacts directly with aquaporin-2 (AQP2), influencing AQP2 trafficking to the apical membrane, which is essential for maintaining water homeostasis in renal epithelial cells. Mice lacking SPA-1 gene exhibit age-dependent T cell immunodeficiency and develop late-onset myeloproliferative disorders, closely resembling human chronic myeloid leukemia, underscoring SPA-1 protein′s importance in immune regulation and cellular signaling pathways.

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SPA-1 Antibody (B-7) References:

1. Rap1 functions as a key regulator of T-cell and antigen-presenting cell interactions and modulates T-cell responses.  |  Katagiri, K., et al. 2002. Mol Cell Biol. 22: 1001-15. PMID: 11809793
2. Antigen-driven T cell anergy and defective memory T cell response via deregulated Rap1 activation in SPA-1-deficient mice.  |  Ishida, D., et al. 2003. Proc Natl Acad Sci U S A. 100: 10919-24. PMID: 12958214
3. Specific recruitment of SPA-1 to the immunological synapse: involvement of actin-bundling protein actinin.  |  Harazaki, M., et al. 2004. Immunol Lett. 92: 221-6. PMID: 15081616
4. Aquaporin-2 trafficking is regulated by PDZ-domain containing protein SPA-1.  |  Noda, Y., et al. 2004. FEBS Lett. 568: 139-45. PMID: 15196935
5. Rap1 and SPA-1 in hematologic malignancy.  |  Kometani, K., et al. 2004. Trends Mol Med. 10: 401-8. PMID: 15310461
6. Molecular mechanisms and drug development in aquaporin water channel diseases: molecular mechanism of water channel aquaporin-2 trafficking.  |  Noda, Y. and Sasaki, S. 2004. J Pharmacol Sci. 96: 249-54. PMID: 15539762
7. Identification of a multiprotein 'motor' complex binding to water channel aquaporin-2.  |  Noda, Y., et al. 2005. Biochem Biophys Res Commun. 330: 1041-7. PMID: 15823548
8. Trafficking mechanism of water channel aquaporin-2.  |  Noda, Y. and Sasaki, S. 2005. Biol Cell. 97: 885-92. PMID: 16293109
9. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression.  |  Hattori, M., et al. 1995. Mol Cell Biol. 15: 552-60. PMID: 7799964

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPA-1 Antibody (B-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SPA-1 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPA-1 Antibody (B-7) Specifications

Alternate Product Name: SPA-1

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 691-990

Epitope Target: SPA-1

Epitope Species: human

Product Reactivity/Detection: SPA-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SPA-1 Antibody (B-7): N/A