Sp4 Antibody (B-1): sc-390124

Sp4 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sp4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Sp4 (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sp4, a member of the Sp transcription factor family, plays a crucial role in regulating gene expression, particularly in the brain, where Sp4 is abundantly expressed. Sp4 is essential for neuronal development and function, influencing the transcription of genes involved in synaptic plasticity and neuronal signaling. Sp transcription factors, including Sp1, Sp2, Sp3, and Sp4, share a highly conserved DNA-binding domain characterized by three zinc fingers at the C-terminus, allowing binding to specific DNA sequences known as GC boxes. While Sp1, Sp3, and Sp4 exhibit a high affinity for these GC boxes, Sp2 has a weaker interaction, binding primarily to GT boxes. Sp4′s unique expression pattern and interactions with other transcription factors underscore Sp4′s importance in the regulation of neuronal genes, making anti-Sp4 antibody (B-1) a valuable tool for researchers studying gene regulation in the nervous system.

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Sp4 Antibody (B-1) References:

1. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression.  |  Kingsley, C. and Winoto, A. 1992. Mol Cell Biol. 12: 4251-61. PMID: 1341900
2. Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes.  |  Hagen, G., et al. 1992. Nucleic Acids Res. 20: 5519-25. PMID: 1454515
3. Regulation of neurotrophin-3 gene transcription by Sp3 and Sp4 in neurons.  |  Ishimaru, N., et al. 2007. J Neurochem. 100: 520-31. PMID: 17059557
4. Association of the Asn306Ser variant of the SP4 transcription factor and an intronic variant in the beta-subunit of transducin with digenic disease.  |  Gao, YQ., et al. 2007. Mol Vis. 13: 287-92. PMID: 17356515
5. Over-representation of potential SP4 target genes within schizophrenia-risk genes.  |  Zhou, X. 2022. Mol Psychiatry. 27: 849-854. PMID: 34750502
6. Coexisting autoantibodies against transcription factor Sp4 are associated with decreased cancer risk in patients with dermatomyositis with anti-TIF1γ autoantibodies.  |  Hosono, Y., et al. 2023. Ann Rheum Dis. 82: 246-252. PMID: 36008132
7. The FOXP4-AS1/miR-3130-3p/SP4 feedback loop is associated with prostate cancer.  |  Gu, T., et al. 2022. Cell Mol Biol (Noisy-le-grand). 68: 161-166. PMID: 37114256
8. SP4 Facilitates Esophageal Squamous Cell Carcinoma Progression by Activating PHF14 Transcription and Wnt/Β-Catenin Signaling.  |  Wei, L., et al. 2024. Mol Cancer Res. 22: 55-69. PMID: 37768180
9. Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3.  |  Hagen, G., et al. 1995. J Biol Chem. 270: 24989-94. PMID: 7559627
10. Sp1-mediated transcriptional activation is repressed by Sp3.  |  Hagen, G., et al. 1994. EMBO J. 13: 3843-51. PMID: 8070411
11. Human Sp3 transcriptional regulator gene (SP3) maps to chromosome 2q31.  |  Kalff-Suske, M., et al. 1996. Genomics. 37: 410-2. PMID: 8938460
12. Sp1 and Sp3 regulate expression of the neuronal nicotinic acetylcholine receptor beta4 subunit gene.  |  Bigger, CB., et al. 1997. J Biol Chem. 272: 25976-82. PMID: 9325332

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sp4 Antibody (B-1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Sp4 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sp4 Antibody (B-1) Specifications

Alternate Product Name: Sp4

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 171-440

Epitope Target: Sp4

Epitope Species: human

Product Reactivity/Detection: Sp4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Sp4 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent