Sp110 CRISPR/Cas9 KO Plasmid (h): sc-405542
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sp110 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sp110 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sp110 Antibody (B-10): sc-376741
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp110 CRISPR/Cas9 KO Plasmid (h) | sc-405542 | 20 µg | $397.00 |
Overview
SP110 encodes Sp110, a nuclear body–associated protein implicated in transcriptional regulation and chromatin-dependent control of immune programs. Sp110 participates in nuclear architecture and interacts with regulatory complexes that shape interferon-responsive and inflammatory gene expression, linking it to host defense pathways and macrophage-associated functions. Genetic and expression studies have connected SP110 to susceptibility and variation in immune-mediated phenotypes, supporting its use as a molecular entry point for dissecting innate immune signaling networks. Altered SP110 activity is also leveraged to study how nuclear body components influence cell state, transcriptional outputs, and pathogen-response gene circuits.
Sp110 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SP110 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SP110 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SP110 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sp110 protein expression.
This CRISPR knockout system enables efficient generation of SP110-deficient cell models for investigation of Sp110 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SP110 exon(s) critical for Sp110 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SP110 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sp110 CRISPR/Cas9 KO Plasmid (h) and Sp110 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SP110 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sp110 HDR Plasmid (h) and Sp110 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SP110 homology arms to support homology-directed repair at defined SP110 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.