Sp110 Antibody (B-10): sc-376741

Sp110 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects human Sp110 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Sp110 Antibody (B-10) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants. Sp110, also known as Speckled 110 kDa, IPR1, VODI, IFI41, or IFI75, is a phosphoprotein belonging to the SP-100/SP140 family of nuclear body components. Sp110 functions as a transcriptional coactivator of nuclear hormone receptors, regulating gene expression in response to hormonal signals. Sp110 monoclonal antibody (B-10) recognizes a protein induced by interferon-gamma and all-trans retinoic acid, contributing to immunoprotective mechanisms against pathogens. Mutations in SP110 can lead to hepatic venoocclusive disease with immunodeficiency (VODI), characterized by T and B cell immunodeficiency, absent tissue plasma cells, absent lymph node germinal centers, and severe hypogammaglobulinemia. Alternative splicing produces five Sp110 isoforms, with isoform 3 (Sp110b) specifically interacting with Hepatitis C core protein, suggesting a role in viral pathogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sp110 Antibody (B-10) References:

1. Sp110 localizes to the PML-Sp100 nuclear body and may function as a nuclear hormone receptor transcriptional coactivator.  |  Bloch, DB., et al. 2000. Mol Cell Biol. 20: 6138-46. PMID: 10913195
2. Role and fate of PML nuclear bodies in response to interferon and viral infections.  |  Regad, T. and Chelbi-Alix, MK. 2001. Oncogene. 20: 7274-86. PMID: 11704856
3. Modulation of retinoid signaling by a cytoplasmic viral protein via sequestration of Sp110b, a potent transcriptional corepressor of retinoic acid receptor, from the nucleus.  |  Watashi, K., et al. 2003. Mol Cell Biol. 23: 7498-509. PMID: 14559998
4. Epstein-Barr virus (EBV) SM protein induces and recruits cellular Sp110b to stabilize mRNAs and enhance EBV lytic gene expression.  |  Nicewonger, J., et al. 2004. J Virol. 78: 9412-22. PMID: 15308735
5. From mice to humans: identification of commonly deregulated genes in mammary cancer via comparative SAGE studies.  |  Hu, Y., et al. 2004. Cancer Res. 64: 7748-55. PMID: 15520179
6. Mutations in the gene encoding the PML nuclear body protein Sp110 are associated with immunodeficiency and hepatic veno-occlusive disease.  |  Roscioli, T., et al. 2006. Nat Genet. 38: 620-2. PMID: 16648851
7. No associations of human pulmonary tuberculosis with Sp110 variants.  |  Thye, T., et al. 2006. J Med Genet. 43: e32. PMID: 16816019
8. An antigen produced by splicing of noncontiguous peptides in the reverse order.  |  Warren, EH., et al. 2006. Science. 313: 1444-7. PMID: 16960008
9. Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells.  |  de la Fuente, J., et al. 2007. BMC Infect Dis. 7: 110. PMID: 17883869

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sp110 Antibody (B-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Sp110 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sp110 Antibody (B-10) Specifications

Alternate Product Name: Sp110

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 216-455

Epitope Target: Sp110

Epitope Species: human

Product Reactivity/Detection: Sp110

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Sp110 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin