Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SP-D CRISPR/Cas9 KO Plasmid (m): sc-422903
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SP-D CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SP-D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SP-D Antibody (1A10A9): sc-53138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SP-D CRISPR/Cas9 KO Plasmid (m) | sc-422903 | 20 µg | $397.00 |
Overview
Sftpd encodes surfactant protein D (SP-D), a secreted collectin produced primarily by alveolar type II cells and Clara cells that contributes to pulmonary surfactant homeostasis and innate immune defense at mucosal surfaces. SP-D recognizes carbohydrate patterns on microbes and apoptotic cells, promoting opsonization, agglutination, and modulation of macrophage and neutrophil activity through lectin-dependent interactions. Functionally, SP-D intersects with pathways controlling alveolar proteostasis, inflammatory cytokine signaling, and oxidative stress responses that shape airway barrier integrity. Altered Sftpd/SP-D activity is linked to dysregulated lung inflammation and remodeling, making it relevant for studying mechanisms of acute lung injury, asthma- and COPD-like phenotypes, and susceptibility to respiratory infection in mouse models.
SP-D CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sftpd gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sftpd together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sftpd open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SP-D protein expression.
This CRISPR knockout system enables efficient generation of Sftpd-deficient cell models for investigation of SP-D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sftpd exon(s) critical for SP-D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sftpd genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SP-D CRISPR/Cas9 KO Plasmid (m) and SP-D CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sftpd locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SP-D HDR Plasmid (m) and SP-D HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sftpd homology arms to support homology-directed repair at defined Sftpd target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.