SP-B Antibody (F-2): sc-133143

SP-B Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects SP-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SP-B Antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SP-B plays a crucial role in pulmonary surfactant function, which is essential for reducing surface tension at the air-liquid interface in the alveoli, thereby preventing alveolar collapse during exhalation and facilitating normal respiration. SP-B is primarily located in the alveolar type II cells and is vital for proper surfactant functioning, as SP-B enhances the stability and spreading of surfactant lipids. Additionally, SP-B interacts with surfactant proteins SP-C and phospholipids, forming a complex that is critical for the biophysical properties of pulmonary surfactant. SP-B′s importance in maintaining lung function underscores SP-B′s relevance in respiratory diseases, where surfactant dysfunction can lead to conditions such as neonatal respiratory distress syndrome and acute respiratory distress syndrome in adults.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SP-B Antibody (F-2) References:

1. Increased metalloproteinase activity, oxidant production, and emphysema in surfactant protein D gene-inactivated mice.  |  Wert, SE., et al. 2000. Proc Natl Acad Sci U S A. 97: 5972-7. PMID: 10801980
2. The pulmonary collectins, SP-A and SP-D, orchestrate innate immunity in the lung.  |  McCormack, FX. and Whitsett, JA. 2002. J Clin Invest. 109: 707-12. PMID: 11901176
3. Human surfactant polypeptide SP-B. Disulfide bridges, C-terminal end, and peptide analysis of the airway form.  |  Johansson, J., et al. 1992. FEBS Lett. 301: 165-7. PMID: 1568474
4. Structures and properties of the surfactant-associated proteins.  |  Hawgood, S. and Shiffer, K. 1991. Annu Rev Physiol. 53: 375-94. PMID: 2042965
5. Structure and expression of the pulmonary surfactant protein SP-C gene in the mouse.  |  Glasser, SW., et al. 1990. J Biol Chem. 265: 21986-91. PMID: 2254341
6. Surfactant protein B gene polymorphism is associated with severe influenza.  |  To, KKW., et al. 2014. Chest. 145: 1237-1243. PMID: 24337193
7. Environmental Pollutant Ozone Causes Damage to Lung Surfactant Protein B (SP-B).  |  Hemming, JM., et al. 2015. Biochemistry. 54: 5185-97. PMID: 26270023
8. The expression of the surfactant proteins SP-A and SP-B during postnatal alveolarization of the rat lung.  |  Roeder, F., et al. 2024. PLoS One. 19: e0297889. PMID: 38483982
9. Molecular and cellular processing of lung surfactant.  |  Rooney, SA., et al. 1994. FASEB J. 8: 957-67. PMID: 8088461
10. Genomic organization of human surfactant protein D (SP-D). SP-D is encoded on chromosome 10q22.2-23.1.  |  Crouch, E., et al. 1993. J Biol Chem. 268: 2976-83. PMID: 8428971
11. Molecular structures and interactions of pulmonary surfactant components.  |  Johansson, J. and Curstedt, T. 1997. Eur J Biochem. 244: 675-93. PMID: 9108235
12. Functional roles of the lung surfactant proteins SP-A and SP-D in innate immunity.  |  Reid, KB. 1998. Immunobiology. 199: 200-7. PMID: 9777406

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SP-B Antibody (F-2) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-SP-B Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SP-B Antibody (F-2) Specifications

Alternate Product Name: SP-B

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: SP-B

Epitope Species: human

Product Reactivity/Detection: SP-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for SP-B Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin