Sox-21 CRISPR/Cas9 KO Plasmid (h): sc-406354
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox-21 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sox-21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox-21 Antibody (2G10): sc-293461
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox-21 CRISPR/Cas9 KO Plasmid (h) | sc-406354 | 20 µg | $397.00 |
Overview
SOX21 encodes the transcription factor Sox-21, a high-mobility group (HMG) box DNA-binding protein that regulates lineage commitment and differentiation programs in ectodermal and neural tissues. Sox-21 participates in transcriptional networks that intersect with Notch and Wnt signaling and can modulate the balance between progenitor maintenance and neuronal maturation through context-dependent gene repression or activation. During development it contributes to cell fate specification and tissue patterning, making it a useful node for studying transcriptional control of neurogenesis and epidermal differentiation. Dysregulated SOX21 expression has been reported in multiple tumor types and in aberrant differentiation states, supporting investigation of its roles in oncogenic transcriptional programs and cell-state transitions.
Sox-21 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOX21 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOX21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOX21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sox-21 protein expression.
This CRISPR knockout system enables efficient generation of SOX21-deficient cell models for investigation of Sox-21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOX21 exon(s) critical for Sox-21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOX21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sox-21 CRISPR/Cas9 KO Plasmid (h) and Sox-21 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOX21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sox-21 HDR Plasmid (h) and Sox-21 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOX21 homology arms to support homology-directed repair at defined SOX21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.