Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sox-13 CRISPR/Cas9 KO Plasmid (h): sc-409568
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox-13 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sox-13 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox-13 Antibody (B-5): sc-515107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox-13 CRISPR/Cas9 KO Plasmid (h) | sc-409568 | 20 µg | $397.00 |
Overview
SOX13 encodes Sox-13, a high-mobility group (HMG) box transcription factor in the SOX family that binds DNA to modulate lineage-specific gene expression programs. Sox-13 participates in developmental and differentiation processes, including regulation of transcriptional networks that shape cell fate decisions and immune-related gene expression. In human cells, SOX13 activity is linked to pathways governing transcriptional control and chromatin-dependent regulation, with reported associations to immune dysregulation and cancer-related transcriptional states in specific contexts. Dissecting SOX13 function helps clarify how SOX-family factors coordinate gene regulatory circuits that influence proliferation, differentiation, and stress-responsive transcription.
Sox-13 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOX13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOX13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOX13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sox-13 protein expression.
This CRISPR knockout system enables efficient generation of SOX13-deficient cell models for investigation of Sox-13 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOX13 exon(s) critical for Sox-13 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOX13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sox-13 CRISPR/Cas9 KO Plasmid (h) and Sox-13 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOX13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sox-13 HDR Plasmid (h) and Sox-13 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOX13 homology arms to support homology-directed repair at defined SOX13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.