Superoxide Dismutase 2/SOD2 Antibody (A-2): sc-133134

SOD-2 Antibody (A-2) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 1-222, representing the full-length Superoxide Dismutase 2 (SOD2) protein of human origin, and is reactive with mouse, rat, and human species. SOD2, also known as manganese superoxide dismutase (MnSOD), is a homotetrameric mitochondrial enzyme composed of four identical subunits, each binding a manganese ion critical for catalytic activity. This structural arrangement enables SOD2 to efficiently convert reactive oxygen species (ROS), such as the superoxide anion, into less harmful molecules like hydrogen peroxide and oxygen, thus protecting cells from oxidative stress. The tetrameric structure not only enhances stability but also optimizes enzymatic activity required to mitigate the damaging effects of ROS, which are byproducts of mitochondrial respiration. Given SOD2′s essential role in maintaining cellular homeostasis, structural alterations or deficiencies are associated with various pathological conditions, including neurodegenerative diseases like Alzheimer′s and Parkinson′s, ischemic heart disease, and idiopathic dilated cardiomyopathy. Anti-SOD2 antibody (A-2) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), making SOD2 (A-2) antibody a versatile tool for detection and study in diverse research applications. SOD2 monoclonal antibody (A-2) is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing researchers with flexibility to explore critical structural and functional properties.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Superoxide Dismutase 2/SOD2 Antibody (A-2) References:

1. Polymorphisms in the SOD2 and HLA-DRB1 genes are associated with nonfamilial idiopathic dilated cardiomyopathy in Japanese.  |  Hiroi, S., et al. 1999. Biochem Biophys Res Commun. 261: 332-9. PMID: 10425186
2. Synthesis and processing of the precursor for human mangano-superoxide dismutase.  |  Wispé, JR., et al. 1989. Biochim Biophys Acta. 994: 30-6. PMID: 2462451
3. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase.  |  Li, Y., et al. 1995. Nat Genet. 11: 376-81. PMID: 7493016
4. DNA typing of HLA class II genes in Japanese patients with dilated cardiomyopathy.  |  Nishi, H., et al. 1995. J Mol Cell Cardiol. 27: 2385-92. PMID: 8576952
5. Human mitochondrial manganese superoxide dismutase polymorphic variant Ile58Thr reduces activity by destabilizing the tetrameric interface.  |  Borgstahl, GE., et al. 1996. Biochemistry. 35: 4287-97. PMID: 8605177
6. A novel neurological phenotype in mice lacking mitochondrial manganese superoxide dismutase.  |  Melov, S., et al. 1998. Nat Genet. 18: 159-63. PMID: 9462746
7. Probing the active site of human manganese superoxide dismutase: the role of glutamine 143.  |  Hsieh, Y., et al. 1998. Biochemistry. 37: 4731-9. PMID: 9537988
8. Mitochondrial disease in superoxide dismutase 2 mutant mice.  |  Melov, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 846-51. PMID: 9927656

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Superoxide Dismutase 2/SOD2 Antibody (A-2) has 100 citations in a variety of scientific publications.

Primary antibodies like Anti-Superoxide Dismutase 2/SOD2 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Superoxide Dismutase 2/SOD2 Antibody (A-2) Specifications

Alternate Product Name: SOD2

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-222

Epitope Target: SOD-2

Epitope Species: human

Product Reactivity/Detection: SOD-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 100 citations in various scientific publications

Available Conjugates/Forms for Superoxide Dismutase 2/SOD2 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin