Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SOCS-6 CRISPR/Cas9 KO Plasmid (h): sc-402344
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SOCS-6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SOCS-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SOCS-6 Antibody (G-3): sc-74597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SOCS-6 CRISPR/Cas9 KO Plasmid (h) | sc-402344 | 20 µg | $397.00 |
Overview
SOCS6 encodes suppressor of cytokine signaling 6 (SOCS-6), a member of the SOCS family that attenuates receptor and cytokine signaling through its SH2 domain and SOCS box–dependent recruitment of ubiquitin ligase complexes. SOCS-6 participates in negative regulation of JAK/STAT and receptor tyrosine kinase pathways, influencing downstream PI3K–AKT and MAPK signaling outputs that control proliferation, differentiation, and survival. By modulating signal duration and promoting turnover of activated signaling components, SOCS-6 contributes to maintenance of cellular homeostasis in immune and growth factor–responsive contexts. Altered SOCS6 expression or function has been associated with dysregulated signaling networks implicated in oncogenic transformation and immune-related phenotypes, making it relevant for mechanistic studies of pathway feedback and signal termination.
SOCS-6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOCS6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOCS6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOCS6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SOCS-6 protein expression.
This CRISPR knockout system enables efficient generation of SOCS6-deficient cell models for investigation of SOCS-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOCS6 exon(s) critical for SOCS-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOCS6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SOCS-6 CRISPR/Cas9 KO Plasmid (h) and SOCS-6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOCS6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SOCS-6 HDR Plasmid (h) and SOCS-6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOCS6 homology arms to support homology-directed repair at defined SOCS6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.