SNX3 Antibody (G-7) recommended for detection of SNX3 of mouse, rat and human origin by WB, IP, IF and ELISA

SNX3 Antibody (G-7): sc-376667

SNX3 Antibody (G-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • Anti-SNX3 Antibody (G-7) is a mouse monoclonal IgG1 (kappa light chain) SNX3 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 122-162 near the C-terminus of SNX3 of human origin
    • Anti-SNX3 Antibody (G-7) is recommended for detection of SNX3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-SNX3 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNX3 (G-7): sc-376667.

Hello, in SNX3 protein what amino acids is this AB really recognizing, since SNX3 is just 162aa, so the epitope between 137-171 is basically the C-terminus. Was the peptide for immunization tagged for purification at the N-terminus?

Asked by: MattK
Thank you for your question The exact peptide sequence used to develop this mouse monoclonal antibody is considered proprietary. We will revise the information for this antibody to more accurately describe the antigen as specific for an epitope mapping between amino acids 122-162 near the C-terminus of SNX3 of human origin.
Answered by: Technical Support
Date published: 2017-11-22

What application is the blocking peptide sc-376667 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good western blotting antibody Clear band on western blotting from cell lysates at 1/1000
Date published: 2017-09-20
Rated 5 out of 5 by from WB I ever used this snx3 antibody for WB (loading 60ug mouse spleen tissue extracts),1:800 for dilution.The target band is as showing on their datasheet at 20kda,very good experimence for WB detection.
Date published: 2017-09-10
Rated 5 out of 5 by from worked fine Used for western blot in 293 cell ( SNX3 stably expressing) out come is good, highly working condition (1:1000), lower dilution can also work fine.
Date published: 2017-08-12
Rated 5 out of 5 by from Clear WB band I got clear WB band of snx3 in primary neonatal mouse cardiomyocytes.
Date published: 2017-08-07
Rated 5 out of 5 by from Produced positive Western blot data of SNX3 Produced positive Western blot data of SNX3 expression in non-transfected and human SNX3 transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-11-11
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