SNAT1 CRISPR/Cas9 KO Plasmid (m): sc-430613
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNAT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNAT1 Antibody (H-9): sc-137032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAT1 CRISPR/Cas9 KO Plasmid (m) | sc-430613 | 20 µg | $397.00 |
Overview
Slc38a1 encodes the sodium-coupled neutral amino acid transporter 1 (SNAT1), a system A transporter that mediates Na⁺-dependent uptake of small neutral amino acids such as glutamine, alanine, and serine across the plasma membrane. By regulating intracellular amino acid availability, SNAT1 contributes to cellular nitrogen balance, redox homeostasis, and metabolic signaling programs including amino acid–sensitive mTORC1 regulation. In mouse tissues, SNAT1 activity is especially relevant to neuronal and glial amino acid handling that supports neurotransmitter cycling and bioenergetic demands. Altered neutral amino acid transport has been linked to metabolic stress responses and neurobiology-associated phenotypes, making Slc38a1 a useful target for studying nutrient transport and signaling crosstalk.
SNAT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc38a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc38a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc38a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNAT1 protein expression.
This CRISPR knockout system enables efficient generation of Slc38a1-deficient cell models for investigation of SNAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc38a1 exon(s) critical for SNAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc38a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNAT1 CRISPR/Cas9 KO Plasmid (m) and SNAT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc38a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNAT1 HDR Plasmid (m) and SNAT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc38a1 homology arms to support homology-directed repair at defined Slc38a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.