SMYD1 Antibody (A-12): sc-514804
- SMYD1 Antibody (A-12) is a mouse monoclonal IgG2b κ SMYD1 antibody provided at 200 µg/ml
- raised against amino acids 181-480 mapping near the C-terminus of SMYD1 of human origin
- SMYD1 Antibody (A-12) is recommended for detection of SMYD1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SMYD1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SMYD1 Antibody (A-12). This work is in progress.
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SMYD1 Antibody (A-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects SMYD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMYD1 (A-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMYD1, also known as SET and MYND domain-containing protein 1, BOP, ZMYND18, or ZMYND22, is a critical nuclear and cytoplasmic protein that plays a vital role in cardiac morphogenesis and cardiomyocyte differentiation. SMYD1 contains one SET domain and one MYND-type zinc finger, which are essential for function as a transcription factor. SMYD1 is specifically expressed in cardiac and skeletal muscle, where SMYD1 interacts with histone deacetylases HDAC1, HDAC2, and HDAC3, functioning as a histone deacetylase-dependent transcriptional repressor. The importance of SMYD1′s structure lies in its ability to modulate gene expression through these interactions, which are crucial for normal cardiac development. Defects or deletions in the gene encoding SMYD1 can lead to impaired maturation of ventricular cardiomyocytes, underscoring its essential role in heart development and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SMYD1 Antibody (A-12) References:
- Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis. | Gottlieb, PD., et al. 2002. Nat Genet. 31: 25-32. PMID: 11923873
- m-Bop, a repressor protein essential for cardiogenesis, interacts with skNAC, a heart- and muscle-specific transcription factor. | Sims, RJ., et al. 2002. J Biol Chem. 277: 26524-9. PMID: 12011100
- BOP, a regulator of right ventricular heart development, is a direct transcriptional target of MEF2C in the developing heart. | Phan, D., et al. 2005. Development. 132: 2669-78. PMID: 15890826
- SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryos. | Tan, X., et al. 2006. Proc Natl Acad Sci U S A. 103: 2713-8. PMID: 16477022
- Muscle-specific expression of the smyd1 gene is controlled by its 5.3-kb promoter and 5'-flanking sequence in zebrafish embryos. | Du, SJ., et al. 2006. Dev Dyn. 235: 3306-15. PMID: 17048253
- Bop: a new T-cell-restricted gene located upstream of and opposite to mouse CD8b. | Hwang, I. and Gottlieb, PD. 1995. Immunogenetics. 42: 353-61. PMID: 7590968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMYD1 Antibody (A-12) | sc-514804 | 200 µg/ml | $316.00 | |||
SMYD1 Antibody (A-12) AC | sc-514804 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SMYD1 Antibody (A-12) HRP | sc-514804 HRP | 200 µg/ml | $316.00 | |||
SMYD1 Antibody (A-12) FITC | sc-514804 FITC | 200 µg/ml | $330.00 | |||
SMYD1 Antibody (A-12) PE | sc-514804 PE | 200 µg/ml | $343.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 488 | sc-514804 AF488 | 200 µg/ml | $357.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 546 | sc-514804 AF546 | 200 µg/ml | $357.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 594 | sc-514804 AF594 | 200 µg/ml | $357.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 647 | sc-514804 AF647 | 200 µg/ml | $357.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 680 | sc-514804 AF680 | 200 µg/ml | $357.00 | |||
SMYD1 Antibody (A-12) Alexa Fluor® 790 | sc-514804 AF790 | 200 µg/ml | $357.00 |