SMS1 Antibody (G-8): sc-133135

SMS1 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMS1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMS1 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMS1, a member of the sphingomyelin synthase family, plays a crucial role in lipid metabolism by converting phosphatidylcholine and ceramide into sphingomyelin and diacylglycerol, and vice versa, depending on substrate concentrations. This bidirectional activity maintains cellular homeostasis and regulates processes such as cell survival and apoptosis. SMS1 is predominantly located in the Golgi apparatus, which is essential for lipid synthesis and transport, while SMS2 is primarily found at the plasma membrane. SMS1 expression is regulated through four alternatively spliced mRNAs, producing three distinct proteins with varying tissue distributions and functional roles. SMS1 is expressed in several key tissues, including liver, muscle, heart, brain, stomach, and kidney, highlighting SMS1′s importance in various physiological processes. SMS1 inhibition by compounds such as tricyclodecan-9-yl-xanthogenate (D609) has been linked to tumor cell cytotoxicity, underscoring SMS1′s potential implications in cancer biology.

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SMS1 Antibody (G-8) References:

1. Identification of a family of animal sphingomyelin synthases.  |  Huitema, K., et al. 2004. EMBO J. 23: 33-44. PMID: 14685263
2. Sphingomyelin synthase as a potential target for D609-induced apoptosis in U937 human monocytic leukemia cells.  |  Meng, A., et al. 2004. Exp Cell Res. 292: 385-92. PMID: 14697345
3. Expression cloning of a human cDNA restoring sphingomyelin synthesis and cell growth in sphingomyelin synthase-defective lymphoid cells.  |  Yamaoka, S., et al. 2004. J Biol Chem. 279: 18688-93. PMID: 14976195
4. The mouse sphingomyelin synthase 1 (SMS1) gene is alternatively spliced to yield multiple transcripts and proteins.  |  Yang, Z., et al. 2005. Gene. 363: 123-32. PMID: 16226406
5. Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation. Does sphingomyelin synthase account for the putative phosphatidylcholine-specific phospholipase C?  |  Luberto, C. and Hannun, YA. 1998. J Biol Chem. 273: 14550-9. PMID: 9603970

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMS1 Antibody (G-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SMS1 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMS1 Antibody (G-8) Specifications

Alternate Product Name: SMS1

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-150

Epitope Target: SMS1

Epitope Species: human

Product Reactivity/Detection: SMS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SMS1 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin