SMRT Antibody (1212): sc-32298

SMRT Antibody (1212) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMRT protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SMRT (1212) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMRT protein, also known as Nuclear Receptor Corepressor 2, plays a crucial role in gene expression regulation by acting as a transcriptional repressor. SMRT (1212) monoclonal antibody recognizes a protein that interacts with retinoic acid receptors (RARs) and thyroid hormone receptors (TRs) in their unliganded state, effectively silencing target genes until ligand binding occurs, which releases SMRT and allows for transcriptional activation. This mechanism is vital for proper vertebrate development and tissue differentiation, ensuring genes are expressed at the right time and context. SMRT protein consists of 1495 amino acids and features distinct receptor-interacting domains at its carboxy terminus, while its amino terminus contains two repressor domains. Notably, SMRT has an 8 amino acid sequence that differentiates it from its extended form, SMRTe, which contains 2514 amino acids and is regulated during the cell cycle, indicating involvement in cycle-specific gene expression across various signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SMRT Antibody (1212) References:

1. SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor.  |  Park, EJ., et al. 1999. Proc Natl Acad Sci U S A. 96: 3519-24. PMID: 10097068
2. Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.  |  Yang, N., et al. 1991. Proc Natl Acad Sci U S A. 88: 3559-63. PMID: 1850832
3. Nuclear receptor corepressor SMRT regulates mitochondrial oxidative metabolism and mediates aging-related metabolic deterioration.  |  Reilly, SM., et al. 2010. Cell Metab. 12: 643-53. PMID: 21109196
4. A functional retinoic acid receptor encoded by the gene on human chromosome 12.  |  Ishikawa, T., et al. 1990. Mol Endocrinol. 4: 837-44. PMID: 2172793
5. Elevated nuclear expression of the SMRT corepressor in breast cancer is associated with earlier tumor recurrence.  |  Smith, CL., et al. 2012. Breast Cancer Res Treat. 136: 253-65. PMID: 23015261
6. PPARα and NCOR/SMRT corepressor network in liver metabolic regulation.  |  Kang, Z. and Fan, R. 2020. FASEB J. 34: 8796-8809. PMID: 32396271
7. Nuclear corepressor SMRT acts as a strong regulator of both β-oxidation and suppressor of fibrosis in the differentiation process of mouse skeletal muscle cells.  |  Shimizu, H., et al. 2022. PLoS One. 17: e0277830. PMID: 36454860
8. Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.  |  Hörlein, AJ., et al. 1995. Nature. 377: 397-404. PMID: 7566114
9. Polarity-specific activities of retinoic acid receptors determined by a co-repressor.  |  Kurokawa, R., et al. 1995. Nature. 377: 451-4. PMID: 7566126
10. A transcriptional co-repressor that interacts with nuclear hormone receptors.  |  Chen, JD. and Evans, RM. 1995. Nature. 377: 454-7. PMID: 7566127
11. Phosphorylation enhances the target gene sequence-dependent dimerization of thyroid hormone receptor with retinoid X receptor.  |  Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMRT Antibody (1212) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-SMRT Antibody (1212) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMRT Antibody (1212) Specifications

Alternate Product Name: NCOR2

Product Clone Name: 1212

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 994-1005

Epitope Target: SMRT

Epitope Species: human

Product Reactivity/Detection: SMRT and SMRTe

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for SMRT Antibody (1212): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SMRTe antibody is SMRTe Antibody (1542/H7): sc-13554.