SMRTe Antibody (1542/H7): sc-13554

SMRTe Antibody (1542/H7) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMRTe protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin embedded sections (IHCP). SMRTe monoclonal antibody (1542/H7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. SMRTe, also known as Nuclear Receptor Corepressor 2, plays a crucial role in the regulation of gene expression by acting as a transcriptional repressor. SMRTe functions in retinoic acid signaling through interactions with retinoic acid receptors (RARs) and thyroid hormone receptors to modulate their activity. SMRTe contains a unique N-terminal sequence spanning over 1,000 amino acids, not present in SMRT. This structural difference suggests distinct regulatory functions in various signaling pathways, particularly during cell cycle progression. SMRTe expression undergoes tight regulation, indicating involvement in cycle-specific gene expression, essential for proper cellular function and development. Research on SMRTe and associated protein interactions provides valuable insights into transcriptional regulation mechanisms and retinoid effects on cellular processes.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SMRTe Antibody (1542/H7) References:

1. SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor.  |  Park, EJ., et al. 1999. Proc Natl Acad Sci U S A. 96: 3519-24. PMID: 10097068
2. SMRTE inhibits MEF2C transcriptional activation by targeting HDAC4 and 5 to nuclear domains.  |  Wu, X., et al. 2001. J Biol Chem. 276: 24177-85. PMID: 11304536
3. Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.  |  Yang, N., et al. 1991. Proc Natl Acad Sci U S A. 88: 3559-63. PMID: 1850832
4. MicroRNAs 10a and 10b are potent inducers of neuroblastoma cell differentiation through targeting of nuclear receptor corepressor 2.  |  Foley, NH., et al. 2011. Cell Death Differ. 18: 1089-98. PMID: 21212796
5. A functional retinoic acid receptor encoded by the gene on human chromosome 12.  |  Ishikawa, T., et al. 1990. Mol Endocrinol. 4: 837-44. PMID: 2172793
6. MicroRNA-184 downregulates nuclear receptor corepressor 2 in mouse spermatogenesis.  |  Wu, J., et al. 2011. BMC Dev Biol. 11: 64. PMID: 22017809
7. The pregnane X receptor (PXR) and the nuclear receptor corepressor 2 (NCoR2) modulate cell growth in head and neck squamous cell carcinoma.  |  Rigalli, JP., et al. 2018. PLoS One. 13: e0193242. PMID: 29470550
8. Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.  |  Hörlein, AJ., et al. 1995. Nature. 377: 397-404. PMID: 7566114
9. Polarity-specific activities of retinoic acid receptors determined by a co-repressor.  |  Kurokawa, R., et al. 1995. Nature. 377: 451-4. PMID: 7566126
10. A transcriptional co-repressor that interacts with nuclear hormone receptors.  |  Chen, JD. and Evans, RM. 1995. Nature. 377: 454-7. PMID: 7566127
11. Phosphorylation enhances the target gene sequence-dependent dimerization of thyroid hormone receptor with retinoid X receptor.  |  Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMRTe Antibody (1542/H7) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SMRTe Antibody (1542/H7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMRTe Antibody (1542/H7) Specifications

Alternate Product Name: NCOR2

Product Clone Name: 1542/H7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 994-1005

Epitope Target: SMRTe

Epitope Species: human

Product Reactivity/Detection: SMRT and SMRTe

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SMRTe Antibody (1542/H7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SMRT antibody is SMRT Antibody (1212): sc-32298.