Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SMPDL3B CRISPR/Cas9 KO Plasmid (m): sc-430315
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SMPDL3B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SMPDL3B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SMPDL3B Antibody (H-3): sc-137113
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMPDL3B CRISPR/Cas9 KO Plasmid (m) | sc-430315 | 20 µg | $397.00 |
Overview
Smpdl3b encodes sphingomyelin phosphodiesterase acid-like 3B (SMPDL3B), a membrane-associated phosphodiesterase implicated in regulating sphingolipid and phospholipid metabolism at the cell surface. SMPDL3B has been linked to modulation of Toll-like receptor signaling, macrophage activation states, and homeostatic control of inflammatory responses through effects on membrane lipid composition and receptor organization. In mouse immune and renal biology, altered SMPDL3B activity has been associated with changes in innate immune responsiveness and podocyte function, connecting lipid remodeling to tissue injury and inflammation. As a result, Smpdl3b is frequently studied in pathways intersecting lipid signaling, endolysosomal trafficking, and immune-cell phenotypes relevant to inflammatory and metabolic disease models.
SMPDL3B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Smpdl3b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Smpdl3b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Smpdl3b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SMPDL3B protein expression.
This CRISPR knockout system enables efficient generation of Smpdl3b-deficient cell models for investigation of SMPDL3B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Smpdl3b exon(s) critical for SMPDL3B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Smpdl3b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SMPDL3B CRISPR/Cas9 KO Plasmid (m) and SMPDL3B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Smpdl3b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SMPDL3B HDR Plasmid (m) and SMPDL3B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Smpdl3b homology arms to support homology-directed repair at defined Smpdl3b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.