SMPDL3B Antibody (H-3): sc-137113
- SMPDL3B Antibody (H-3) is a mouse monoclonal IgG1 κ SMPDL3B antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 196-271 mapping within an internal region of SMPDL3B of mouse origin
- SMPDL3B Antibody (H-3) is recommended for detection of SMPDL3B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SMPDL3B Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SMPDL3B Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SMPDL3B Antibody (H-3) (see ordering information below).
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SMPDL3B Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMPDL3B of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SMPDL3B (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMPDL3B, also known as sphingomyelin phosphodiesterase, acid-like 3B, or ASMLPD, is a 455 amino acid secreted protein that plays a crucial role in lipid metabolism and cell signaling. SMPDL3B is primarily expressed in granulosa cells of the ovarian follicle and is a homolog of acid sphingomyelinase (ASM), a lysosomal enzyme responsible for hydrolyzing sphingomyelin into ceramide and phosphocholine. ASM enzymatic activity is vital for regulating apoptosis in germ cell lines, and deficiencies in ASM are linked to severe conditions such as type A and type B Niemann-Pick disease. Type A is a devastating neurodegenerative disorder that manifests in infancy, often leading to death by age three, while type B presents with a later onset and is characterized by non-neuropathic symptoms. SMPDL3B′s ability to interact with various lipid substrates underscores its importance in cellular processes, making anti-SMPDL3B antibody (H-3) an essential tool for researchers studying lipid metabolism and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SMPDL3B Antibody (H-3) References:
- Transcription factors Sp1 and AP-2 mediate induction of acid sphingomyelinase during monocytic differentiation. | Langmann, T., et al. 1999. J Lipid Res. 40: 870-80. PMID: 10224156
- Identification and expression of five mutations in the human acid sphingomyelinase gene causing types A and B Niemann-Pick disease. Molecular evidence for genetic heterogeneity in the neuronopathic and non-neuronopathic forms. | Takahashi, T., et al. 1992. J Biol Chem. 267: 12552-8. PMID: 1618760
- Generation of biologically active linear and cyclic peptides has revealed a unique fine specificity of rituximab and its possible cross-reactivity with acid sphingomyelinase-like phosphodiesterase 3b precursor. | Perosa, F., et al. 2006. Blood. 107: 1070-7. PMID: 16223774
- Epigenetic control of the critical region for premature ovarian failure on autosomal genes translocated to the X chromosome: a hypothesis. | Rizzolio, F., et al. 2007. Hum Genet. 121: 441-50. PMID: 17265046
- Human acid sphingomyelinase. Isolation, nucleotide sequence and expression of the full-length and alternatively spliced cDNAs. | Schuchman, EH., et al. 1991. J Biol Chem. 266: 8531-9. PMID: 1840600
- Niemann-Pick disease: a frequent missense mutation in the acid sphingomyelinase gene of Ashkenazi Jewish type A and B patients. | Levran, O., et al. 1991. Proc Natl Acad Sci U S A. 88: 3748-52. PMID: 2023926
- Acid sphingomyelinase from human urine: purification and characterization. | Quintern, LE., et al. 1987. Biochim Biophys Acta. 922: 323-36. PMID: 2825797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMPDL3B Antibody (H-3) | sc-137113 | 200 µg/ml | $316.00 | |||
SMPDL3B Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-528863 | 200 µg Ab; 10 µg BP | $354.00 | |||
SMPDL3B Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-521429 | 200 µg Ab, 40 µg BP | $354.00 | |||
SMPDL3B Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-543175 | 200 µg Ab; 20 µg BP | $354.00 | |||
SMPDL3B Antibody (H-3) AC | sc-137113 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SMPDL3B Antibody (H-3) HRP | sc-137113 HRP | 200 µg/ml | $316.00 | |||
SMPDL3B Antibody (H-3) FITC | sc-137113 FITC | 200 µg/ml | $330.00 | |||
SMPDL3B Antibody (H-3) PE | sc-137113 PE | 200 µg/ml | $343.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 488 | sc-137113 AF488 | 200 µg/ml | $357.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 546 | sc-137113 AF546 | 200 µg/ml | $357.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 594 | sc-137113 AF594 | 200 µg/ml | $357.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 647 | sc-137113 AF647 | 200 µg/ml | $357.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 680 | sc-137113 AF680 | 200 µg/ml | $357.00 | |||
SMPDL3B Antibody (H-3) Alexa Fluor® 790 | sc-137113 AF790 | 200 µg/ml | $357.00 |