Smooth Muscle Actin Antibody (B4): sc-53142

Smooth Muscle Actin Antibody (B4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Smooth Muscle Actin in mouse, rat, human, and chicken samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Smooth Muscle Actin antibody (B4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smooth muscle actin plays a critical role in muscle contraction and cellular motility, serving as a key component of the contractile apparatus in smooth muscle cells to regulate various physiological processes such as blood vessel constriction and gastrointestinal motility. Smooth muscle actin maintains structural integrity and function of smooth muscle tissues, with expression tightly regulated during development and in response to various stimuli. Smooth muscle actin contributes to stress fiber formation, crucial for maintaining cell shape and facilitating cellular movement. Understanding smooth muscle actin dynamics and interactions with other proteins, such as myosin, helps elucidate mechanisms underlying smooth muscle function and disease pathophysiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Smooth Muscle Actin Antibody (B4) References:

1. Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.  |  Sawtell, NM. and Lessard, JL. 1989. J Cell Biol. 109: 2929-37. PMID: 2687290
2. α-Smooth Muscle Actin and ACTA2 Gene Expressions in Vasculopathies.  |  Yuan, SM. 2015. Braz J Cardiovasc Surg. 30: 644-9. PMID: 26934405
3. The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling.  |  Shinde, AV., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 298-309. PMID: 27825850
4. Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents.  |  Samoilenko, T., et al. 2023. Int J Mol Sci. 24: PMID: 38003728
5. Claudin 7 suppresses invasion and metastasis through repression of a smooth muscle actin program.  |  West, JJ., et al. 2024. J Cell Biol. 223: PMID: 39320351
6. Role of microtubule-associated proteins in the control of microtubule assembly.  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
7. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
8. A discourse on modeling F-actin.  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
9. Actin cytoskeleton. Setting the pace of cell movement.  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
10. The origins and evolution of eukaryotic proteins.  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
11. Elastic fibres are an essential component of human placental stem villous stroma and an integrated part of the perivascular contractile sheath.  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
12. Actin bundles in human hair follicles as revealed by confocal laser microscopy.  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smooth Muscle Actin Antibody (B4) has 263 citations in a variety of scientific publications.

Primary antibodies like Anti-Smooth Muscle Actin Antibody (B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smooth Muscle Actin Antibody (B4) Specifications

Alternate Product Name: Alpha Smooth Muscle Actin

Product Clone Name: B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Actin

Epitope Species: chicken

Product Reactivity/Detection: enteric smooth muscle γ-Actin and, to a lesser extent, α-smooth muscle, α-skeletal and α-cardiac (sarcomeric) isoforms of Actin

Species Reactivity/Detection: mouse, rat, human, chicken

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 263 citations in various scientific publications

Available Conjugates/Forms for Smooth Muscle Actin Antibody (B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Smooth Muscle Actin antibody is Smooth Muscle Actin Antibody (CGA7): sc-53015.