SMG1 Antibody (E-4): sc-374557
- SMG1 Antibody (E-4) is a mouse monoclonal IgG1 κ SMG1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 3358-3657 mapping at the C-terminus of SMG1 of human origin
- SMG1 Antibody (E-4) is recommended for detection of SMG1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-SMG1 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374557 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SMG1 Antibody (E-4) for WB applications. These reagents are now offered in bundles with SMG1 Antibody (E-4) (see ordering information below).
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SMG1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMG1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMG1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMG1 protein, also known as ATX or LIP, is a crucial serine/threonine protein kinase composed of 3,657 amino acids, which is expressed in various tissues such as heart and skeletal muscle. SMG1 plays a vital role in mRNA surveillance and cellular response to genotoxic stress, utilizing manganese as a cofactor to catalyze the phosphorylation of target proteins. The phosphorylation of proteins on serine and threonine residues is essential for regulating numerous cellular functions, including cell division, homeostasis, and apoptosis, making SMG1 integral to maintaining cellular integrity and function. Additionally, SMG1 contains one FAT domain, one FATC domain, one HEAT repeat, and one PI3K domain, which contribute to structural and functional diversity. Notably, multiple isoforms of SMG1 arise from alternative splicing events, further enhancing functional repertoire in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SMG1 Antibody (E-4) References:
- Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein. | Denning, G., et al. 2001. J Biol Chem. 276: 22709-14. PMID: 11331269
- Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay. | Yamashita, A., et al. 2001. Genes Dev. 15: 2215-28. PMID: 11544179
- The mRNA surveillance protein hSMG-1 functions in genotoxic stress response pathways in mammalian cells. | Brumbaugh, KM., et al. 2004. Mol Cell. 14: 585-98. PMID: 15175154
- The ATM-related kinase, hSMG-1, bridges genome and RNA surveillance pathways. | Abraham, RT. 2004. DNA Repair (Amst). 3: 919-25. PMID: 15279777
- SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans. | Grimson, A., et al. 2004. Mol Cell Biol. 24: 7483-90. PMID: 15314158
- Distant N- and C-terminal domains are required for intrinsic kinase activity of SMG-1, a critical component of nonsense-mediated mRNA decay. | Morita, T., et al. 2007. J Biol Chem. 282: 7799-808. PMID: 17229728
- A protective role for the human SMG-1 kinase against tumor necrosis factor-alpha-induced apoptosis. | Oliveira, V., et al. 2008. J Biol Chem. 283: 13174-84. PMID: 18326048
- hSMG-1 and ATM sequentially and independently regulate the G1 checkpoint during oxidative stress. | Gehen, SC., et al. 2008. Oncogene. 27: 4065-74. PMID: 18332866
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMG1 Antibody (E-4) | sc-374557 | 200 µg/ml | $322.00 | |||
SMG1 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-527478 | 200 µg Ab; 10 µg BP | $361.00 | |||
SMG1 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-532851 | 200 µg Ab; 20 µg BP | $361.00 | |||
SMG1 Antibody (E-4) X | sc-374557 X | 200 µg/0.1 ml | $322.00 | |||
SMG1 Antibody (E-4) AC | sc-374557 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SMG1 Antibody (E-4) HRP | sc-374557 HRP | 200 µg/ml | $322.00 | |||
SMG1 Antibody (E-4) FITC | sc-374557 FITC | 200 µg/ml | $336.00 | |||
SMG1 Antibody (E-4) PE | sc-374557 PE | 200 µg/ml | $349.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 488 | sc-374557 AF488 | 200 µg/ml | $364.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 546 | sc-374557 AF546 | 200 µg/ml | $364.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 594 | sc-374557 AF594 | 200 µg/ml | $364.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 647 | sc-374557 AF647 | 200 µg/ml | $364.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 680 | sc-374557 AF680 | 200 µg/ml | $364.00 | |||
SMG1 Antibody (E-4) Alexa Fluor® 790 | sc-374557 AF790 | 200 µg/ml | $364.00 |