SmcY CRISPR/Cas9 KO Plasmid (h2): sc-403217-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SmcY CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SmcY genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SmcY Antibody (4C6): sc-293280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SmcY CRISPR/Cas9 KO Plasmid (h2) | sc-403217-KO-2 | 20 µg | $397.00 |
Overview
KDM5D encodes the Y chromosome–linked lysine demethylase SmcY, a JmjC-domain enzyme that removes H3K4me3/me2 marks to regulate transcriptional programs and chromatin accessibility. By reshaping promoter-associated histone methylation, SmcY contributes to epigenetic control of cell-state transitions, lineage-restricted gene expression, and coordination of DNA-templated processes such as replication and repair. Variation or dysregulation of KDM5 family activity has been linked to altered differentiation and proliferation phenotypes, and KDM5D is frequently studied in the context of male-specific gene regulation and Y-linked contributions to disease biology. As an epigenetic modifier, SmcY provides a tractable entry point for dissecting how histone methylation dynamics influence signaling outputs and genome regulation in human cells.
SmcY CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the KDM5D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KDM5D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KDM5D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SmcY protein expression.
This CRISPR knockout system enables efficient generation of KDM5D-deficient cell models for investigation of SmcY signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KDM5D exon(s) critical for SmcY function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KDM5D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SmcY CRISPR/Cas9 KO Plasmid (h) and SmcY CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KDM5D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SmcY HDR Plasmid (h) and SmcY HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KDM5D homology arms to support homology-directed repair at defined KDM5D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.