SMAP1 Antibody (D-3): sc-390553
- SMAP1 Antibody (D-3) is a mouse monoclonal IgG2a κ SMAP1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 313-339 of SMAP1 of human origin
- SMAP1 Antibody (D-3) is recommended for detection of SMAP1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-SMAP1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SMAP1 Antibody (D-3) for WB applications. These reagents are now offered in bundles with SMAP1 Antibody (D-3) (see ordering information below).
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SMAP1 Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects SMAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMAP1 Antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMAP1, also known as stromal membrane-associated protein 1 or small ArfGAP 1, is a 467 amino acid peripheral membrane protein that plays a crucial role in clathrin-dependent endocytosis by localizing to the cytoplasmic side of the cell membrane. As a GTPase-activating protein for ARF6, SMAP1 is essential for regulating membrane trafficking and cellular signaling pathways, which are vital for maintaining cellular homeostasis and responding to external stimuli. SMAP1 is widely expressed in various tissues, including lymph node, spinal cord, bone marrow, adrenal gland, trachea, stomach, thyroid, and embryonic hematopoietic tissues. SMAP1 contains one Arf-GAP domain and exists in multiple isoforms due to alternative splicing events, with SMAP1 gene located on human chromosome 6q13. This chromosome encompasses 170 million base pairs, accounting for nearly 6% of the human genome, and is associated with various conditions, including Parkinson′s disease, Stickler syndrome, and a susceptibility to bipolar disorder.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SMAP1 Antibody (D-3) References:
- Cellular and molecular regulation of an erythropoietic inductive microenvironment (EIM). | Obinata, M. and Yanai, N. 1999. Cell Struct Funct. 24: 171-9. PMID: 10532351
- Cloning, characterization and chromosome mapping of the human SMAP1 gene. | Marcos, I., et al. 2002. Gene. 292: 167-71. PMID: 12119110
- Parkin, a gene implicated in autosomal recessive juvenile parkinsonism, is a candidate tumor suppressor gene on chromosome 6q25-q27. | Cesari, R., et al. 2003. Proc Natl Acad Sci U S A. 100: 5956-61. PMID: 12719539
- A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis. | Tanabe, K., et al. 2005. Mol Biol Cell. 16: 1617-28. PMID: 15659652
- Mutation screening of three candidate genes, ELOVL5, SMAP1 and GLULD1 in autosomal recessive retinitis pigmentosa. | Barragan, I., et al. 2005. Int J Mol Med. 16: 1163-7. PMID: 16273301
- A Stickler syndrome gene is linked to chromosome 6 near the COL11A2 gene. | Brunner, HG., et al. 1994. Hum Mol Genet. 3: 1561-4. PMID: 7833911
- Involvement of stromal membrane-associated protein (SMAP-1) in erythropoietic microenvironment. | Sato, Y., et al. 1998. J Biochem. 124: 209-16. PMID: 9644265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMAP1 Antibody (D-3) | sc-390553 | 200 µg/ml | $316.00 | |||
SMAP1 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-530188 | 200 µg Ab; 10 µg BP | $354.00 | |||
SMAP1 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-523581 | 200 µg Ab, 40 µg BP | $354.00 | |||
SMAP1 Antibody (D-3) AC | sc-390553 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SMAP1 Antibody (D-3) HRP | sc-390553 HRP | 200 µg/ml | $316.00 | |||
SMAP1 Antibody (D-3) FITC | sc-390553 FITC | 200 µg/ml | $330.00 | |||
SMAP1 Antibody (D-3) PE | sc-390553 PE | 200 µg/ml | $343.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 488 | sc-390553 AF488 | 200 µg/ml | $357.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 546 | sc-390553 AF546 | 200 µg/ml | $357.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 594 | sc-390553 AF594 | 200 µg/ml | $357.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 647 | sc-390553 AF647 | 200 µg/ml | $357.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 680 | sc-390553 AF680 | 200 µg/ml | $357.00 | |||
SMAP1 Antibody (D-3) Alexa Fluor® 790 | sc-390553 AF790 | 200 µg/ml | $357.00 | |||
SMAP1 (D-3) Neutralizing Peptide | sc-390553 P | 100 µg/0.5 ml | $68.00 |