Sm G Antibody (A-11): sc-398741
- Sm G Antibody (A-11) is a mouse monoclonal IgG1 κ Sm G antibody provided at 200 µg/ml
- raised against amino acids 1-76 representing full length Sm G of human origin
- Sm G Antibody (A-11) is recommended for detection of Sm G and Sm G-like of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Sm G Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Sm G Antibody (A-11) for WB applications. These reagents are now offered in bundles with Sm G Antibody (A-11) (see ordering information below).
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Sm G Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sm G protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Sm G antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sm G protein, also known as SNRPG, plays a crucial role in the assembly and function of small nuclear ribonucleoproteins (snRNPs), which are essential components of the spliceosome responsible for pre-mRNA splicing in eukaryotic cells. Proper functioning of the spliceosome is vital for gene expression regulation, as this process ensures accurate removal of introns and joining of exons, ultimately influencing protein diversity and cellular function. Sm G, along with other Sm proteins, forms a stable core complex that binds to the conserved Sm site on U snRNA, facilitating the formation of functional snRNPs. Genes encoding Sm G and its interacting partner Sm F are located on chromosomes 2 and 12, respectively, and both are expressed as 0.5 kb transcripts in HeLa cells. Sm proteins are significant in the context of autoimmune diseases, as these proteins are frequently targeted by autoantibodies in patients with systemic lupus erythematosus, highlighting their importance in both cellular biology and clinical research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sm G Antibody (A-11) References:
- U2 RNA shares a structural domain with U1, U4, and U5 RNAs. | Branlant, C., et al. 1982. EMBO J. 1: 1259-65. PMID: 6202507
- snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions. | Hermann, H., et al. 1995. EMBO J. 14: 2076-88. PMID: 7744013
- The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro. | Raker, VA., et al. 1996. EMBO J. 15: 2256-69. PMID: 8641291
- A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states. | Brahms, H., et al. 1997. Arthritis Rheum. 40: 672-82. PMID: 9125249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sm G Antibody (A-11) | sc-398741 | 200 µg/ml | $322.00 | |||
Sm G Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-530912 | 200 µg Ab; 10 µg BP | $361.00 | |||
Sm G Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-524466 | 200 µg Ab, 40 µg BP | $361.00 | |||
Sm G Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-544315 | 200 µg Ab; 20 µg BP | $361.00 | |||
Sm G Antibody (A-11) AC | sc-398741 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Sm G Antibody (A-11) HRP | sc-398741 HRP | 200 µg/ml | $322.00 | |||
Sm G Antibody (A-11) FITC | sc-398741 FITC | 200 µg/ml | $336.00 | |||
Sm G Antibody (A-11) PE | sc-398741 PE | 200 µg/ml | $349.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 488 | sc-398741 AF488 | 200 µg/ml | $364.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 546 | sc-398741 AF546 | 200 µg/ml | $364.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 594 | sc-398741 AF594 | 200 µg/ml | $364.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 647 | sc-398741 AF647 | 200 µg/ml | $364.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 680 | sc-398741 AF680 | 200 µg/ml | $364.00 | |||
Sm G Antibody (A-11) Alexa Fluor® 790 | sc-398741 AF790 | 200 µg/ml | $364.00 |