SLU7 Antibody (B-11): sc-376985
- SLU7 Antibody (B-11) is a mouse monoclonal IgG1 κ SLU7 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 241-420 mapping within an internal region of SLU7 of human origin
- SLU7 Antibody (B-11) is recommended for detection of SLU7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-SLU7 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376985 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SLU7 Antibody (B-11) for WB and IHC(P) applications. These reagents are now offered in bundles with SLU7 Antibody (B-11) (see ordering information below).
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SLU7 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SLU7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SLU7 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SLU7 plays a critical role in the mRNA splicing process, specifically during the second catalytic step where SLU7 is essential for the accurate selection of the adenine-guanine (AG) dinucleotide at the 3′ splice site. This function is vital because improper splicing can lead to the production of dysfunctional proteins, which may contribute to various diseases, including cancer. SLU7, the human homolog of the yeast step II splice factor Slu7, associates with the spliceosome late in the splicing pathway, ensuring that exon 1 is held tightly within the spliceosome for the precise attack on the prespecified AG. SLU7 depletion in HeLa nuclear extracts has demonstrated SLU7′s necessity for maintaining the integrity of the splicing process, highlighting SLU7′s importance in gene expression regulation and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SLU7 Antibody (B-11) References:
- Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing. | Chua, K. and Reed, R. 1999. Genes Dev. 13: 841-50. PMID: 10197984
- The RNA splicing factor hSlu7 is required for correct 3' splice-site choice. | Chua, K. and Reed, R. 1999. Nature. 402: 207-10. PMID: 10647016
- How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. | James, SA., et al. 2002. RNA. 8: 1068-77. PMID: 12212850
- An essential splicing factor, SLU7, mediates 3' splice site choice in yeast. | Frank, D. and Guthrie, C. 1992. Genes Dev. 6: 2112-24. PMID: 1427075
- Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. | Aronova, A., et al. 2007. RNA. 13: 1437-44. PMID: 17626844
- SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. | Ansari, A. and Schwer, B. 1995. EMBO J. 14: 4001-9. PMID: 7664739
- Requirement for SLU7 in yeast pre-mRNA splicing is dictated by the distance between the branchpoint and the 3' splice site. | Brys, A. and Schwer, B. 1996. RNA. 2: 707-17. PMID: 8756413
- Functional and physical interaction between the yeast splicing factors Slu7 and Prp18. | Zhang, X. and Schwer, B. 1997. Nucleic Acids Res. 25: 2146-52. PMID: 9153314
- Mechanical devices of the spliceosome: motors, clocks, springs, and things. | Staley, JP. and Guthrie, C. 1998. Cell. 92: 315-26. PMID: 9476892
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLU7 Antibody (B-11) | sc-376985 | 200 µg/ml | $322.00 | |||
SLU7 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-527568 | 200 µg Ab; 10 µg BP | $361.00 | |||
SLU7 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-532941 | 200 µg Ab; 20 µg BP | $361.00 | |||
SLU7 Antibody (B-11) X | sc-376985 X | 200 µg/0.1 ml | $322.00 | |||
SLU7 Antibody (B-11) AC | sc-376985 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SLU7 Antibody (B-11) HRP | sc-376985 HRP | 200 µg/ml | $322.00 | |||
SLU7 Antibody (B-11) FITC | sc-376985 FITC | 200 µg/ml | $336.00 | |||
SLU7 Antibody (B-11) PE | sc-376985 PE | 200 µg/ml | $349.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 488 | sc-376985 AF488 | 200 µg/ml | $364.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 546 | sc-376985 AF546 | 200 µg/ml | $364.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 594 | sc-376985 AF594 | 200 µg/ml | $364.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 647 | sc-376985 AF647 | 200 µg/ml | $364.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 680 | sc-376985 AF680 | 200 µg/ml | $364.00 | |||
SLU7 Antibody (B-11) Alexa Fluor® 790 | sc-376985 AF790 | 200 µg/ml | $364.00 |