Slp2 CRISPR/Cas9 KO Plasmid (h): sc-405977
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Slp2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Slp2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Slp2 Antibody (C-4): sc-393847
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slp2 CRISPR/Cas9 KO Plasmid (h) | sc-405977 | 20 µg | $397.00 |
Overview
SYTL2 encodes synaptotagmin-like protein 2 (Slp2), a Rab effector that links secretory vesicles to the plasma membrane through C2 domain–mediated phospholipid interactions and Rab-binding activity. Slp2 contributes to regulated exocytosis and vesicle docking/priming events that influence membrane trafficking, receptor turnover, and compartmentalized secretion in diverse cell types. Through its role in Rab-dependent trafficking, SYTL2 can impact signaling dynamics, cytoskeletal organization, and cell motility programs. Altered expression or function of vesicle trafficking regulators such as SYTL2 has been associated with dysregulated secretion and invasive phenotypes in cancer and other diseases where membrane transport pathways are remodeled.
Slp2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SYTL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SYTL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SYTL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Slp2 protein expression.
This CRISPR knockout system enables efficient generation of SYTL2-deficient cell models for investigation of Slp2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SYTL2 exon(s) critical for Slp2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SYTL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Slp2 CRISPR/Cas9 KO Plasmid (h) and Slp2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SYTL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Slp2 HDR Plasmid (h) and Slp2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SYTL2 homology arms to support homology-directed repair at defined SYTL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.