Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SLC26A3 CRISPR/Cas9 KO Plasmid (h): sc-403891
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC26A3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC26A3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLC26A3 Antibody (H-8): sc-376187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC26A3 CRISPR/Cas9 KO Plasmid (h) | sc-403891 | 20 µg | $397.00 |
Overview
SLC26A3 encodes a membrane anion exchanger (also known as DRA) that mediates electroneutral Cl⁻/HCO₃⁻ exchange at the apical surface of epithelial cells, with prominent roles in intestinal fluid and electrolyte absorption. By regulating bicarbonate secretion and chloride uptake, SLC26A3 contributes to luminal pH control and epithelial transport processes that interface with CFTR-dependent ion handling and mucosal homeostasis. Disruption or dysregulation of SLC26A3 has been linked to congenital chloride diarrhea and broader phenotypes involving epithelial barrier function and inflammatory signaling in the gastrointestinal tract. Its expression and activity are therefore relevant to studies of epithelial differentiation, transport physiology, and inflammation-associated remodeling of the gut microenvironment.
SLC26A3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC26A3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC26A3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC26A3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC26A3 protein expression.
This CRISPR knockout system enables efficient generation of SLC26A3-deficient cell models for investigation of SLC26A3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC26A3 exon(s) critical for SLC26A3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC26A3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC26A3 CRISPR/Cas9 KO Plasmid (h) and SLC26A3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC26A3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC26A3 HDR Plasmid (h) and SLC26A3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC26A3 homology arms to support homology-directed repair at defined SLC26A3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.