Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SLC13A5 CRISPR/Cas9 KO Plasmid (h): sc-407118
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC13A5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC13A5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLC13A5 Antibody (2G4): sc-293277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC13A5 CRISPR/Cas9 KO Plasmid (h) | sc-407118 | 20 µg | $397.00 |
Overview
SLC13A5 encodes a sodium-dependent citrate transporter (NaCT) that mediates cellular uptake of citrate, linking extracellular citrate availability to intracellular carbon flux. By influencing cytosolic citrate pools, SLC13A5 supports acetyl-CoA generation for de novo lipogenesis and protein acetylation, and can modulate energy sensing networks that coordinate glycolysis, TCA cycle anaplerosis, and redox balance. SLC13A5 activity is most studied in metabolically active tissues and neurons, where citrate transport intersects with mitochondrial metabolism and epigenetic regulation via acetylation-dependent pathways. Genetic perturbation of SLC13A5 has been associated with neurometabolic dysfunction and altered lipid and glucose homeostasis, making it relevant for mechanistic studies of metabolic and neuronal phenotypes.
SLC13A5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC13A5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC13A5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC13A5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC13A5 protein expression.
This CRISPR knockout system enables efficient generation of SLC13A5-deficient cell models for investigation of SLC13A5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC13A5 exon(s) critical for SLC13A5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC13A5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC13A5 CRISPR/Cas9 KO Plasmid (h) and SLC13A5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC13A5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC13A5 HDR Plasmid (h) and SLC13A5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC13A5 homology arms to support homology-directed repair at defined SLC13A5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.