SLAM Antibody (E-11): sc-166939

SLAM Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SLAM of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SLAM (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SLAM, also known as signaling lymphocyte-activation molecule or CDw150, plays a crucial role in T cell activation and proliferation by providing a costimulatory signal that is independent of the CD28 pathway. This is particularly important in the immune response, as SLAM enhances T cell expansion and lymphokine production following T cell receptor engagement with antigen. Additionally, SLAM interacts with SLAM-associated protein (SAP), which contains an SH2 domain that inhibits the recruitment of SH-PTP2 to the SLAM docking site, a process that is regulated by Fyn phosphorylation of SLAM . Notably, mutations in the SAP gene have been linked to X-linked lymphoproliferative disease (XLP), highlighting the significance of SLAM in maintaining immune system integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SLAM Antibody (E-11) References:

1. Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.  |  Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
2. Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: from pro-B to plasma cells.  |  De Salort, J., et al. 2011. Immunol Lett. 134: 129-36. PMID: 20933013
3. Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers.  |  Larochelle, A., et al. 2011. Blood. 117: 1550-4. PMID: 21163926
4. The role of the SLAM-SAP signaling pathway in the modulation of CD4+ T cell responses.  |  Vilar, ML., et al. 2011. Braz J Med Biol Res. 44: 276-82. PMID: 21445533
5. SLAM family receptors in natural killer cells - Mediators of adhesion, activation and inhibition via cis and trans interactions.  |  Claus, M., et al. 2019. Clin Immunol. 204: 37-42. PMID: 30359773
6. NK cell recognition of hematopoietic cells by SLAM-SAP families.  |  Chen, S. and Dong, Z. 2019. Cell Mol Immunol. 16: 452-459. PMID: 30911116
7. SLAM-SAP-Fyn: Old Players with New Roles in iNKT Cell Development and Function.  |  Bahal, D., et al. 2019. Int J Mol Sci. 20: PMID: 31569599
8. A novel receptor involved in T-cell activation.  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
9. SLAM and its role in T cell activation and Th cell responses.  |  Aversa, G., et al. 1997. Immunol Cell Biol. 75: 202-5. PMID: 9107577
10. Engagement of the signaling lymphocytic activation molecule (SLAM) on activated T cells results in IL-2-independent, cyclosporin A-sensitive T cell proliferation and IFN-gamma production.  |  Aversa, G., et al. 1997. J Immunol. 158: 4036-44. PMID: 9126961
11. The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.  |  Sayos, J., et al. 1998. Nature. 395: 462-9. PMID: 9774102
12. Effector pathways regulating T cell activation.  |  Favero, J. and Lafont, V. 1998. Biochem Pharmacol. 56: 1539-47. PMID: 9973174

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SLAM Antibody (E-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SLAM Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SLAM Antibody (E-11) Specifications

Alternate Product Name: SLAM

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-110

Epitope Target: SLAM

Epitope Species: human

Product Reactivity/Detection: SLAM

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SLAM Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin