Skp2 p45 Antibody (A-2): sc-74477

Skp2 p45 Antibody (A-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Skp2 p45 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Skp2 p45 (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Skp2 p45 plays a pivotal role in regulating cell cycle progression, particularly in G1 to S phase transition, by targeting specific cyclins for degradation. Skp2 p45 primarily resides in the nucleus, interacting with cyclin-dependent kinases and other regulatory proteins to facilitate timely cell cycle progression. Precise nuclear localization of Skp2 p45 enables effective regulation of cyclin levels, maintaining cellular homeostasis and preventing uncontrolled cell proliferation. Dysregulation of Skp2 p45 has been implicated in various cancers, highlighting Skp2 p45′s importance as a potential therapeutic target. Using anti-Skp2 p45 antibody (A-2) in research provides valuable insights into cell cycle regulation mechanisms and Skp2 p45′s role in cellular processes.

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Skp2 p45 Antibody (A-2) References:

1. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.  |  Lees, E., et al. 1992. Genes Dev. 6: 1874-85. PMID: 1398067
2. Co-purification of p34cdc2/p58cyclin A proline-directed protein kinase and the retinoblastoma tumor susceptibility gene product: interaction of an oncogenic serine/threonine protein kinase with a tumor-suppressor protein.  |  Williams, RT., et al. 1992. Oncogene. 7: 423-32. PMID: 1532245
3. Interaction between human cyclin A and adenovirus E1A-associated p107 protein.  |  Faha, B., et al. 1992. Science. 255: 87-90. PMID: 1532458
4. Cyclin A and the retinoblastoma gene product complex with a common transcription factor.  |  Bandara, LR., et al. 1991. Nature. 352: 249-51. PMID: 1830372
5. Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma.  |  Wang, J., et al. 1990. Nature. 343: 555-7. PMID: 1967822
6. Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.  |  Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
7. Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.  |  Draetta, G., et al. 1989. Cell. 56: 829-38. PMID: 2538242
8. A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.  |  Giordano, A., et al. 1989. Cell. 58: 981-90. PMID: 2570639
9. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy.  |  Shin, HJ., et al. 2016. Nature. 534: 553-7. PMID: 27309807
10. Skp2 in the ubiquitin-proteasome system: A comprehensive review.  |  Asmamaw, MD., et al. 2020. Med Res Rev. 40: 1920-1949. PMID: 32391596
11. MYOD-SKP2 axis boosts tumorigenesis in fusion negative rhabdomyosarcoma by preventing differentiation through p57Kip2 targeting.  |  Pomella, S., et al. 2023. Nat Commun. 14: 8373. PMID: 38102140
12. p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase.  |  Zhang, H., et al. 1995. Cell. 82: 915-25. PMID: 7553852

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Skp2 p45 Antibody (A-2) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-Skp2 p45 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Skp2 p45 Antibody (A-2) Specifications

Alternate Product Name: Skp2 p45

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-435

Epitope Target: Skp2 p45

Epitope Species: human

Product Reactivity/Detection: Skp2 p45

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for Skp2 p45 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent