SKIP Antibody (B-6): sc-365362

SKIP Antibody (B-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects SKIP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SKIP (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SKIP, also known as skeletal muscle and kidney enriched inositol phosphatase, plays a crucial role in cellular signaling by selectively dephosphorylating phosphatidylinositol 4,5-bisphosphate, which is essential for generating second messengers in response to extracellular signals. SKIP is predominantly expressed in skeletal muscle, heart, brain, and kidney, and SKIP expression in these tissues is vital for regulating various physiological processes, including muscle contraction and neuronal signaling. SKIP localizes in the cytoplasm and at ruffling membranes, where SKIP influences the actin cytoskeletal structure; cells expressing SKIP exhibit a loss of actin stress fibers, indicating a negative regulatory role in cytoskeletal dynamics. SKIP interactions with other signaling molecules further underscore SKIP′s importance in maintaining cellular homeostasis and responding to environmental cues.

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SKIP Antibody (B-6) References:

1. Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles.  |  Mochizuki, Y. and Takenawa, T. 1999. J Biol Chem. 274: 36790-5. PMID: 10593988
2. Identification and characterization of a novel inositol polyphosphate 5-phosphatase.  |  Ijuin, T., et al. 2000. J Biol Chem. 275: 10870-5. PMID: 10753883
3. Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation.  |  Gurung, R., et al. 2003. J Biol Chem. 278: 11376-85. PMID: 12536145
4. SKIP negatively regulates insulin-induced GLUT4 translocation and membrane ruffle formation.  |  Ijuin, T. and Takenawa, T. 2003. Mol Cell Biol. 23: 1209-20. PMID: 12556481
5. The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP protein.  |  Stegmann, CM., et al. 2010. PLoS One. 5: e10013. PMID: 20368803
6. Regulation of insulin signaling and glucose transporter 4 (GLUT4) exocytosis by phosphatidylinositol 3,4,5-trisphosphate (PIP3) phosphatase, skeletal muscle, and kidney enriched inositol polyphosphate phosphatase (SKIP).  |  Ijuin, T. and Takenawa, T. 2012. J Biol Chem. 287: 6991-9. PMID: 22247557
7. Role of phosphatidylinositol 3,4,5-trisphosphate (PIP3) 5-phosphatase skeletal muscle- and kidney-enriched inositol polyphosphate phosphatase (SKIP) in myoblast differentiation.  |  Ijuin, T. and Takenawa, T. 2012. J Biol Chem. 287: 31330-41. PMID: 22815484
8. Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78.  |  Ijuin, T., et al. 2015. Biochim Biophys Acta. 1853: 3192-201. PMID: 26376412
9. Phosphatidylinositol 3,4,5-Trisphosphate Phosphatase SKIP Links Endoplasmic Reticulum Stress in Skeletal Muscle to Insulin Resistance.  |  Ijuin, T., et al. 2016. Mol Cell Biol. 36: 108-18. PMID: 26483413
10. Regulation of second messengers by the inositol polyphosphate 5-phosphatases.  |  Mitchell, CA., et al. 1996. Biochem Soc Trans. 24: 994-1000. PMID: 8968499
11. Phosphatidylinositol signalling reactions.  |  Zhang, X. and Majerus, PW. 1998. Semin Cell Dev Biol. 9: 153-60. PMID: 9599410
12. The diversity and possible functions of the inositol polyphosphate 5-phosphatases.  |  Erneux, C., et al. 1998. Biochim Biophys Acta. 1436: 185-99. PMID: 9838104

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SKIP Antibody (B-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SKIP Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SKIP Antibody (B-6) Specifications

Alternate Product Name: SKIP

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 419-443

Epitope Target: SKIP

Epitope Species: human

Product Reactivity/Detection: SKIP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SKIP Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin