Skeletal Muscle Myosin Antibody (S58): sc-32733

Skeletal Muscle Myosin Antibody (S58) is a mouse monoclonal IgA kappa light chain antibody that detects the slow fiber-specific Skeletal Muscle Myosin heavy chain of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Skeletal Muscle Myosin antibody (S58) is available as the non-conjugated form. Myosin plays a crucial role in muscle contraction and cellular movement, functioning as a motor protein that interacts with Actin to generate force. Skeletal Muscle Myosin (S58) monoclonal antibody recognizes a hexameric protein composed of two heavy chain subunits and light chain subunits, which can be either phosphorylatable or non-phosphorylatable. This structural arrangement allows for precise regulation of muscle contraction, with different isoforms of myosin heavy chain being expressed based on specific functional demands of muscle tissue. The myosin heavy chains are encoded by the MYH gene family and exhibit Actin-activated ATPase activity, which is essential for motor function. Understanding myosin structure directly influences the speed and efficiency of muscle contraction, making this protein a key factor in muscle physiology and pathology.

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Skeletal Muscle Myosin Antibody (S58) References:

1. Noninvasive Assessment of Skeletal Muscle Myosin Heavy Chain Expression in Trained and Untrained Men.  |  Fry, AC., et al. 2017. J Strength Cond Res. 31: 2355-2362. PMID: 28820846
2. Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats.  |  Yamada, T., et al. 2018. Front Physiol. 9: 1111. PMID: 30147660
3. Cardiac and Skeletal Muscle Myosin Exert Procoagulant Effects.  |  Coleman, JR., et al. 2019. Shock. 52: 554-555. PMID: 31613865
4. Novel blood coagulation molecules: Skeletal muscle myosin and cardiac myosin.  |  Deguchi, H., et al. 2021. J Thromb Haemost. 19: 7-19. PMID: 32920971
5. Single Residue Variation in Skeletal Muscle Myosin Enables Direct and Selective Drug Targeting for Spasticity and Muscle Stiffness.  |  Gyimesi, M., et al. 2020. Cell. 183: 335-346.e13. PMID: 33035452
6. Skeletal muscle myosin and cardiac myosin attenuate heparin's antithrombin-dependent anticoagulant activity.  |  Morla, S., et al. 2021. J Thromb Haemost. 19: 470-477. PMID: 33176060
7. Skeletal muscle myosin promotes coagulation by binding factor XI via its A3 domain and enhancing thrombin-induced factor XI activation.  |  Morla, S., et al. 2022. J Biol Chem. 298: 101567. PMID: 35007530
8. Pseudo-phosphorylation of essential light chains affects the functioning of skeletal muscle myosin.  |  Yampolskaya, DS., et al. 2023. Biophys Chem. 292: 106936. PMID: 36436358
9. Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.  |  Hitachi, K., et al. 2023. FASEB J. 37: e22692. PMID: 36515178
10. Physical activity impacts resting skeletal muscle myosin conformation and lowers its ATP consumption.  |  Lewis, CTA., et al. 2023. J Gen Physiol. 155: PMID: 37227464

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Skeletal Muscle Myosin Antibody (S58) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Skeletal Muscle Myosin Antibody (S58) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Skeletal Muscle Myosin Antibody (S58) Specifications

Alternate Product Name: Skeletal Muscle Myosin

Product Clone Name: S58

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids:

Epitope Target: Myosin heavy chain

Epitope Species: white Leghorn chicken

Product Reactivity/Detection: slow fiber–specific Skeletal Muscle Myosin heavy chain

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Skeletal Muscle Myosin Antibody (S58): N/A

The preferred MYH7 antibody is MYH7 Antibody (A4.951): sc-53090.