Skeletal Muscle Myosin Antibody (F59): sc-32732

Skeletal Muscle Myosin Antibody (F59) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize fast fiber–specific skeletal muscle myosin heavy chains, including MYH1, MYH2, MYH4, and MYH6, across multiple species such as mouse, rat, human, and avian origins. Anti-Skeletal Muscle Myosin antibody (F59) is raised against myosin purified from the pectoralis muscle of adult White Leghorn chickens in applications like western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Skeletal muscle myosin, a highly conserved and ubiquitously expressed motor protein, plays a critical role in muscle contraction by interacting with actin to generate the force necessary for cellular movement. As a key member of the myosin heavy chain family encoded by the MYH gene family, MYH2 possesses actin-activated ATPase activity, which is essential for its motor function and the regulation of muscle contraction speed. Understanding and detecting MYH2 is vital for research into muscle physiology, disease mechanisms, and developmental biology, as different myosin isoforms are differentially expressed based on muscle functional activity. Skeletal Muscle Myosin (F59) monoclonal antibody is available in both non-conjugated form and a variety of conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for diverse experimental requirements. Anti-Skeletal Muscle Myosin antibody (F59) is recommended for scientists investigating the molecular dynamics of skeletal muscle function and pathology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Skeletal Muscle Myosin Antibody (F59) References:

1. Spatial and temporal changes in myosin heavy chain gene expression in skeletal muscle development.  |  Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
2. Myosin isoforms, muscle fiber types, and transitions.  |  Pette, D. and Staron, RS. 2000. Microsc Res Tech. 50: 500-9. PMID: 10998639
3. Identification of three developmentally controlled isoforms of human myosin heavy chains.  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
4. Smooth muscle myosin is composed of homodimeric heavy chains.  |  Kelley, CA., et al. 1992. J Biol Chem. 267: 2127-30. PMID: 1733920
5. Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA.  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
6. Modulation of Skeletal Muscle Contraction by Myosin Phosphorylation.  |  Vandenboom, R. 2016. Compr Physiol. 7: 171-212. PMID: 28135003
7. Physical activity impacts resting skeletal muscle myosin conformation and lowers its ATP consumption.  |  Lewis, CTA., et al. 2023. J Gen Physiol. 155: PMID: 37227464
8. Characterization of a human perinatal myosin heavy-chain transcript.  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
9. Regulation of differentiation of vascular smooth muscle cells.  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
10. Phylogenetic analysis of the myosin superfamily.  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
11. Mechanisms of smooth muscle contraction.  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
12. The mammalian myosin heavy chain gene family.  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Skeletal Muscle Myosin Antibody (F59) has 68 citations in a variety of scientific publications.

Primary antibodies like Anti-Skeletal Muscle Myosin Antibody (F59) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Skeletal Muscle Myosin Antibody (F59) Specifications

Alternate Product Name: Myosin Heavy Chain 2

Product Clone Name: F59

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: myosin heavy chain

Epitope Species: white Leghorn chicken

Product Reactivity/Detection: fast fiber–specific skeletal muscle myosin heavy chain

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for Skeletal Muscle Myosin Antibody (F59): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin