Skeletal Muscle Myosin Antibody (F59) recommended for detection of fast fiber–specific skeletal muscle myosin heavy chain of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)

Skeletal Muscle Myosin Antibody (F59): sc-32732

Skeletal Muscle Myosin Antibody (F59) is rated 4.8 out of 5 by 10.
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Datasheets

    • Anti-Skeletal Muscle Myosin Antibody (F59) is a mouse monoclonal IgG1 κ Skeletal Muscle Myosin antibody, cited in 38 publications, provided at 200 µg/ml
    • raised against Myosin purified from pectoralis muscle of adult white Leghorn chicken origin
    • Anti-Skeletal Muscle Myosin Antibody (F59) is recommended for detection of fast fiber–specific skeletal muscle myosin heavy chain of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
    • Anti-Skeletal Muscle Myosin Antibody (F59) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Skeletal Muscle Myosin (F59): sc-32732.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Skeletal Muscle Myosin Antibody (F59) for WB and IHC(P) applications. This reagent is now offered in a bundle with Skeletal Muscle Myosin Antibody (F59) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Does this antibody detect any specific myosin heavy chain? Such as MYH1, MYH6, MYH7, MYH15, MYH2, MYH3, MYH4 etc?

Asked by: Eric Spinetti
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. This clone is recommended for the detection of fast fiber–specific skeletal muscle myosin heavy chain. The exact myosin heavy chain genes/proteins that are targeted are not known.
Answered by: Technical Support
Date published: 2019-09-20

I am interested in performing a immunoprecipitation of MYH6 using this antibody. However, I don't see any validation of this technique in your website or in any cited papers. Do you have any data demonstrating the efficiency of F59 (sc-32732) for IP?

Asked by: Anonymous
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-09-20

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Good signal This antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 4 out of 5 by from Great features! The antibody is work well in WB at 1:100 of C2C12 cells.
Date published: 2017-08-31
Rated 5 out of 5 by from good WB dilution 1/1000, mouse heart tissue detected clean band
Date published: 2017-05-09
Rated 5 out of 5 by from Sehr gut Application in mouse cells, very clear immunolabelling.
Date published: 2017-04-05
Rated 5 out of 5 by from Outstanding Strong western blot data, low background; nice IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Positive result in WB I bought it for years,tested it in WB with pig dorsal muscles,it is very wonderful
Date published: 2016-12-13
Rated 5 out of 5 by from Western blot and IF publications using mouse Western blot and IF publications using mouse C2C12 myoblasts. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Publishable IHC data in mouse cardiomyocytes Publishable IHC data in mouse cardiomyocytes. -SCBT Publication Review
Date published: 2015-02-08
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