Ska2 Antibody (H-2): sc-514495
- Ska2 Antibody (H-2) is a mouse monoclonal IgG1 κ Ska2 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 16-31 near the N-terminus of Ska2 of human origin
- Ska2 Antibody (H-2) is recommended for detection of Ska2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Ska2 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ska2 Antibody (H-2) for WB applications. These reagents are now offered in bundles with Ska2 Antibody (H-2) (see ordering information below).
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Ska2 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ska2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Ska2 antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ska2, also known as spindle and kinetochore associated complex subunit 2 or FAM33A, is a crucial 121 amino acid component of the Ska1 complex, which plays a vital role in the microtubule-binding subcomplex of the outer kinetochore essential for accurate chromosome segregation. The Ska1 complex is integral to the kinetochore-microtubule interface, directly interacting with microtubules as oligomeric assemblies. Located at the outer kinetochore and spindle microtubules during cell division, Ska2 is indispensable for spindle checkpoint silencing and the transition out of mitosis. Notably, Ska2′s function is critical because downregulation can lead to delayed recruitment of MAD2, a key component of the mitotic spindle checkpoint, to several kinetochores, which may result in the loss of individual chromosomes from the metaphase plate. Ska2 is encoded by a gene situated on human chromosome 17, a region that encompasses over 2.5% of the human genome and is responsible for encoding more than 1,200 genes, highlighting Ska2′s significance in cellular processes and genomic stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ska2 Antibody (H-2) References:
- Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2. | Hanisch, A., et al. 2006. EMBO J. 25: 5504-15. PMID: 17093495
- The MAD1 adhesin of Metarhizium anisopliae links adhesion with blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to plants. | Wang, C. and St Leger, RJ. 2007. Eukaryot Cell. 6: 808-16. PMID: 17337634
- Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor. | Rice, L., et al. 2008. J Endocrinol. 198: 499-509. PMID: 18583474
- Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. | Daub, H., et al. 2008. Mol Cell. 31: 438-48. PMID: 18691976
- The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility. | Welburn, JP., et al. 2009. Dev Cell. 16: 374-85. PMID: 19289083
- Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3. | Gaitanos, TN., et al. 2009. EMBO J. 28: 1442-52. PMID: 19360002
- Large-scale proteomics analysis of the human kinome. | Oppermann, FS., et al. 2009. Mol Cell Proteomics. 8: 1751-64. PMID: 19369195
- Connecting with Ska, a key complex at the kinetochore-microtubule interface. | Guimaraes, GJ. and Deluca, JG. 2009. EMBO J. 28: 1375-7. PMID: 19458608
- Ska3 is required for spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis. | Daum, JR., et al. 2009. Curr Biol. 19: 1467-72. PMID: 19646878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ska2 Antibody (H-2) | sc-514495 | 200 µg/ml | $316.00 | |||
Ska2 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-527909 | 200 µg Ab; 10 µg BP | $354.00 | |||
Ska2 Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-533282 | 200 µg Ab; 20 µg BP | $354.00 | |||
Ska2 Antibody (H-2) AC | sc-514495 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Ska2 Antibody (H-2) HRP | sc-514495 HRP | 200 µg/ml | $316.00 | |||
Ska2 Antibody (H-2) FITC | sc-514495 FITC | 200 µg/ml | $330.00 | |||
Ska2 Antibody (H-2) PE | sc-514495 PE | 200 µg/ml | $343.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 488 | sc-514495 AF488 | 200 µg/ml | $357.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 546 | sc-514495 AF546 | 200 µg/ml | $357.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 594 | sc-514495 AF594 | 200 µg/ml | $357.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 647 | sc-514495 AF647 | 200 µg/ml | $357.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 680 | sc-514495 AF680 | 200 µg/ml | $357.00 | |||
Ska2 Antibody (H-2) Alexa Fluor® 790 | sc-514495 AF790 | 200 µg/ml | $357.00 | |||
Ska2 (H-2) Neutralizing Peptide | sc-514495 P | 100 µg/0.5 ml | $68.00 |