Six4 Antibody (D-5): sc-390779

Six4 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Six4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Six4 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Six4, also known as sine oculis homeobox homolog 4 or AREC3, is a 760 amino acid nuclear protein that plays a crucial role in myogenesis and neurogenesis, as well as the development of various organs. Six4 belongs to the Six/Sine oculis homeobox family, which is characterized by a conserved DNA-binding domain that allows these transcription factors to regulate gene expression during development. Six4 contains a single Six domain, which serves as a homeobox DNA-binding motif, and exhibits approximately 90% sequence similarity with its mouse counterpart, indicating that both proteins likely share similar DNA-binding properties. Proper functioning of Six4 is essential for normal developmental processes, and dysregulation has been implicated in various developmental disorders, making Six4 a significant target for research in developmental biology and related fields.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Six4 Antibody (D-5) References:

1. Structure and chromosome mapping of the human SIX4 and murine Six4 genes.  |  Ozaki, H., et al. 1999. Cytogenet Cell Genet. 87: 108-12. PMID: 10640827
2. Six4, a putative myogenin gene regulator, is not essential for mouse embryonal development.  |  Ozaki, H., et al. 2001. Mol Cell Biol. 21: 3343-50. PMID: 11313460
3. Six1 and Six4 homeoproteins are required for Pax3 and Mrf expression during myogenesis in the mouse embryo.  |  Grifone, R., et al. 2005. Development. 132: 2235-49. PMID: 15788460
4. Slc12a2 is a direct target of two closely related homeobox proteins, Six1 and Six4.  |  Ando, Z., et al. 2005. FEBS J. 272: 3026-41. PMID: 15955062
5. Six1 and Six4 promote survival of sensory neurons during early trigeminal gangliogenesis.  |  Konishi, Y., et al. 2006. Brain Res. 1116: 93-102. PMID: 16938278
6. Live imaging of Drosophila gonad formation reveals roles for Six4 in regulating germline and somatic cell migration.  |  Clark, IB., et al. 2007. BMC Dev Biol. 7: 52. PMID: 17517128
7. Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development.  |  Kawakami, K., et al. 1996. Nucleic Acids Res. 24: 303-10. PMID: 8628654
8. Tissue and developmental distribution of Six family gene products.  |  Ohto, H., et al. 1998. Int J Dev Biol. 42: 141-8. PMID: 9551859

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Six4 Antibody (D-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Six4 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Six4 Antibody (D-5) Specifications

Alternate Product Name: Six4

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 482-781

Epitope Target: Six4

Epitope Species: human

Product Reactivity/Detection: Six4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Six4 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Six1/2/4 antibody is Six1/2/4 Antibody (E-11): sc-398193.