Six3 Antibody (A-1): sc-398797

Six3 Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Six3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Six3 (A-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Six3 is a crucial member of the sine oculis homeodomain transcription factor family, characterized by a conserved DNA-binding domain that plays a vital role in eye and brain development. Six3 is essential for specification and proliferation of the eye field in vertebrates, and Six3 expression is critical during early embryonic development, beginning as early as five to seven weeks of gestation in humans. Six3 is predominantly localized in the developing retina, found in ganglion cells and the inner nuclear layer by 20 weeks of gestation. Six3 maps to human chromosome 2p16-p21, a region associated with significant developmental disorders, including holoprosencephaly type 2 and Malattia leventinese, both exhibiting ocular phenotypes. Six3 regulation of gene expression during critical periods of development underscores its importance in both normal physiology and the etiology of certain congenital conditions.

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Six3 Antibody (A-1) References:

1. Direct interaction of geminin and Six3 in eye development.  |  Del Bene, F., et al. 2004. Nature. 427: 745-9. PMID: 14973488
2. Coexpression of NOR1 and SIX3 proteins in extraskeletal myxoid chondrosarcomas without detectable NR4A3 fusion genes.  |  Hisaoka, M., et al. 2004. Cancer Genet Cytogenet. 152: 101-7. PMID: 15262426
3. First occurrence of aprosencephaly/atelencephaly and holoprosencephaly in a family with a SIX3 gene mutation and phenotype/genotype correlation in our series of SIX3 mutations.  |  Pasquier, L., et al. 2005. J Med Genet. 42: e4. PMID: 15635066
4. Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting Bmp4 expression.  |  Gestri, G., et al. 2005. Development. 132: 2401-13. PMID: 15843413
5. Expression analysis of SIX3 and SIX6 in human tissues reveals differences in expression and a novel correlation between the expression of SIX3 and the genes encoding isocitrate dehyhrogenase and cadherin 18.  |  Aijaz, S., et al. 2005. Genomics. 86: 86-99. PMID: 15953543
6. Comparative analysis of Six 3 and Six 6 distribution in the developing and adult mouse brain.  |  Conte, I., et al. 2005. Dev Dyn. 234: 718-25. PMID: 15973738
7. Pax6-dependence of Six3, Eya1 and Dach1 expression during lens and nasal placode induction.  |  Purcell, P., et al. 2005. Gene Expr Patterns. 6: 110-8. PMID: 16024294
8. Agonistic and antagonistic action of AP2, Msx2, Pax6, Prox1 AND Six3 in the regulation of Sox2 expression.  |  Lengler, J., et al. 2005. Ophthalmic Res. 37: 301-9. PMID: 16118513
9. Haploinsufficiency of Six3 fails to activate Sonic hedgehog expression in the ventral forebrain and causes holoprosencephaly.  |  Geng, X., et al. 2008. Dev Cell. 15: 236-47. PMID: 18694563
10. Six3 cooperates with Hedgehog signaling to specify ventral telencephalon by promoting early expression of Foxg1a and repressing Wnt signaling.  |  Carlin, D., et al. 2012. Development. 139: 2614-24. PMID: 22736245
11. Reduced Nephron Endowment in Six2-TGCtg Mice Is Due to Six3 Misexpression by Aberrant Enhancer-Promoter Interactions in the Transgene.  |  Perl, AJ., et al. 2024. J Am Soc Nephrol. 35: 566-577. PMID: 38447671
12. Genomic cloning, structure, expression pattern, and chromosomal location of the human SIX3 gene.  |  Granadino, B., et al. 1999. Genomics. 55: 100-5. PMID: 9889003

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Six3 Antibody (A-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Six3 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Six3 Antibody (A-1) Specifications

Alternate Product Name: Six3

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 269-327

Epitope Target: Six3

Epitope Species: human

Product Reactivity/Detection: Six3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Six3 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent