Six2 Antibody (H-4): sc-377193

Six2 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Six2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Six2 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Six2 plays a crucial role in kidney development and is involved in the regulation of nephron progenitor cells, which are essential for proper kidney formation and function. Six2 expression is predominantly observed in fetal tissues, highlighting its importance during embryonic development, while limited presence in adult tissues suggests a tightly regulated role that, when disrupted, may contribute to developmental disorders or diseases. Additionally, Six proteins, including Six2, interact with other transcription factors, such as Six4 and Six5, which can bind to the same DNA sequences, indicating a collaborative regulatory network that influences gene expression critical for organogenesis. Understanding Six2 function and interactions is vital for elucidating the mechanisms underlying kidney development and potential pathologies associated with dysregulation.

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Six2 Antibody (H-4) References:

1. The transcription factor Six2 activates expression of the Gdnf gene as well as its own promoter.  |  Brodbeck, S., et al. 2004. Mech Dev. 121: 1211-22. PMID: 15327782
2. Homeoprotein Six2 promotes breast cancer metastasis via transcriptional and epigenetic control of E-cadherin expression.  |  Wang, CA., et al. 2014. Cancer Res. 74: 7357-70. PMID: 25348955
3. Temporally Distinct Six2-Positive Second Heart Field Progenitors Regulate Mammalian Heart Development and Disease.  |  Zhou, Z., et al. 2017. Cell Rep. 18: 1019-1032. PMID: 28122228
4. Six2 Plays an Intrinsic Role in Regulating Proliferation of Mesenchymal Cells in the Developing Palate.  |  Okello, DO., et al. 2017. Front Physiol. 8: 955. PMID: 29218017
5. Crucial and Overlapping Roles of Six1 and Six2 in Craniofacial Development.  |  Liu, Z., et al. 2019. J Dent Res. 98: 572-579. PMID: 30905259
6. De novo SIX2 activation in human kidneys treated with neonatal kidney stem/progenitor cells.  |  Arcolino, FO., et al. 2022. Am J Transplant. 22: 2791-2803. PMID: 35913414
7. HOX genes in human cancers.  |  Cillo, C. Invasion Metastasis. 14: 38-49. PMID: 7657531
8. Defective G2 checkpoint function in cells from individuals with familial cancer syndromes.  |  Paules, RS., et al. 1995. Cancer Res. 55: 1763-73. PMID: 7712486
9. Identification and expression of six family genes in mouse retina.  |  Kawakami, K., et al. 1996. FEBS Lett. 393: 259-63. PMID: 8814301
10. Fission yeast rad12+ regulates cell cycle checkpoint control and is homologous to the Bloom's syndrome disease gene.  |  Davey, S., et al. 1998. Mol Cell Biol. 18: 2721-8. PMID: 9566891
11. Abrogation of the G2 cell cycle checkpoint associated with overexpression of HSIX1: a possible mechanism of breast carcinogenesis.  |  Ford, HL., et al. 1998. Proc Natl Acad Sci U S A. 95: 12608-13. PMID: 9770533
12. Expression of myogenin during embryogenesis is controlled by Six/sine oculis homeoproteins through a conserved MEF3 binding site.  |  Spitz, F., et al. 1998. Proc Natl Acad Sci U S A. 95: 14220-5. PMID: 9826681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Six2 Antibody (H-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Six2 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Six2 Antibody (H-4) Specifications

Alternate Product Name: Six2

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-230

Epitope Target: Six2

Epitope Species: human

Product Reactivity/Detection: Six2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Six2 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent