SIRT1 CRISPR/Cas9 KO Plasmid transfected: sc-400085. Western blot analysis of human SIRT1 CRISPR/Cas9 KO Plasmid expression in empty vector control (A) and SIRT1 CRISPR/Cas9 KO Plasmid transfected (B) 293T whole cell lysates. Blot probed with SIRT1 (B-7): sc-74465. α-actinin (H-12): sc-17829 used as specificity and loading control.
SIRT1 CRISPR/Cas9 KO Plasmid (h): sc-400085
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIRT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SIRT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- SIRT1 HDR Plasmid (h) (sc-400085-HDR) is recommended for co-transfection with SIRT1 CRISPR/Cas9 KO Plasmid (h) to enable selection of successfully edited cells through HDR-mediated integration of a puromycin resistance cassette and RFP reporter gene
- SIRT1 HDR Plasmid (h) is a pool of plasmids, each containing a homology-directed repair (HDR) template corresponding to the gRNA target sites in the SIRT1 CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two ~800 bp homology arms flanking the puromycin resistance and RFP cassettes, designed to bind genomic DNA sequences surrounding the Cas9-induced double-strand break site and facilitate precise HDR-mediated integration
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIRT1 Antibody (B-10): sc-74504
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRT1 CRISPR/Cas9 KO Plasmid (h) | sc-400085 | 20 µg | $397.00 | |||
SIRT1 HDR Plasmid (h) | sc-400085-HDR | 20 µg | $445.00 |