SIRT1 CRISPR Plasmids (h) human SIRT1-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

SIRT1 CRISPR Plasmids (h)

SIRT1 CRISPR Knockout and Activation Products (h) is rated 5.0 out of 5 by 4.
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Datasheets

    • Target species: human; for corresponding mouse product, see SIRT1 CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of SIRT1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using SIRT1 Antibody (B-10): sc-74504
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanSIRT12341110q21.3NM_001142498, NM_001314049, NM_012238Q96EB6604479
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Rated 5 out of 5 by from Works Great !!! I have used in the mammalian cell line and it worked fine !!!
Date published: 2017-03-20
Rated 5 out of 5 by from Have tested this reagent with several different Have tested this reagent with several different cell lines and have been very pleased with the results
Date published: 2015-04-21
Rated 5 out of 5 by from SIRT1 knockout was verified by Western blot SIRT1 knockout was verified by Western blot using sc-74465. Single strong band in CRISPR control. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Worked great in Western blot analysis Worked great in Western blot analysis, complete knockout of SIRT1 in HEK 293T whole cell lysates. -SCBT QC
Date published: 2015-03-11
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