SIRP-α/β1 Antibody (C-8): sc-373896
- SIRP-α/β1 Antibody (C-8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 22-53 near the N-terminus of SIRP-α of human origin
- recommended for detection of SIRP-α and SIRP-β1 of human origin by WB, IP, IF and ELISA
- See SIRP-α/β (A-1): sc-17803 for SIRP-α/β1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SIRP-α/β1 Antibody (C-8) for WB applications. These reagents are now offered in bundles with SIRP-α/β1 Antibody (C-8) (see ordering information below).
SIRP-α/β1 Antibody (C-8) is a mouse monoclonal IgG1 antibody that detects SIRP-α/β1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SIRP-α/β1 is part of the signal-regulatory protein family, which plays a crucial role in modulating immune responses by interacting with CD47 protein, often referred to as the "don′t eat me" signal. This interaction helps prevent phagocytosis of healthy cells by macrophages, maintaining tissue homeostasis and regulating immune surveillance. SIRP-α features an extracellular domain with three immunoglobulin-like structures and a cytoplasmic region containing multiple tyrosine phosphorylation sites, essential for signal transduction. In phosphorylated state, SIRP-α can bind to SH-PTP2, influencing various cellular responses to growth factors and oncogenes. SIRP-β1 variant, while sharing significant sequence homology with SIRP-α, lacks the cytoplasmic domain, indicating a divergence in function and regulatory mechanisms within the SIRP family. Anti-SIRP-α/β1 antibody (C-8) serves as a valuable tool for researchers studying immune regulation and molecular pathways involved in cell signaling and phagocytosis.
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SIRP-α/β1 Antibody (C-8) References:
- SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration. | Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
- OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele. | Hatherley, D., et al. 2016. Monoclon Antib Immunodiagn Immunother. 35: 57-9. PMID: 26871367
- Measles virus nucleocapsid protein modulates the Signal Regulatory Protein-β1 (SIRPβ1) to enhance osteoclast differentiation in Paget's disease of bone. | Sundaram, K., et al. 2017. Bone Rep. 7: 26-32. PMID: 28840181
- Insights into CD47/SIRPα axis-targeting tumor immunotherapy. | Zhang, X., et al. 2018. Antib Ther. 1: 37-42. PMID: 34056543
- Loss of myeloid cell-specific SIRPα, but not CD47, attenuates inflammation and suppresses atherosclerosis. | Singla, B., et al. 2022. Cardiovasc Res. 118: 3097-3111. PMID: 34940829
- Anticancer efficacy of monotherapy with antibodies to SIRPα/SIRPβ1 mediated by induction of antitumorigenic macrophages. | Sakamoto, M., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 34949714
- Thrombospondin-1, CD47, and SIRPα display cell-specific molecular signatures in human islets and pancreata. | Erdem, N., et al. 2023. Am J Physiol Endocrinol Metab. 324: E347-E357. PMID: 36791324
- Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models. | Saito, Y., et al. 2023. Front Immunol. 14: 1294814. PMID: 38162643
- An Insertion Within SIRPβ1 Shows a Dual Effect Over Alzheimer's Disease Cognitive Decline Altering the Microglial Response. | García-Alberca, JM., et al. 2024. J Alzheimers Dis. 98: 601-618. PMID: 38427484
- Metabolic reprograming mediated by tumor cell-intrinsic type I IFN signaling is required for CD47-SIRPα blockade efficacy. | Zhou, H., et al. 2024. Nat Commun. 15: 5759. PMID: 38982116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-α/β1 Antibody (C-8) | sc-373896 | 200 µg/ml | $322.00 | |||
SIRP-α/β1 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-537884 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIRP-α/β1 Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-535333 | 200 µg Ab; 40 µg BP | $361.00 | |||
SIRP-α/β1 Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-545428 | 200 µg Ab; 20 µg BP | $361.00 | |||
SIRP-α/β1 (C-8) Neutralizing Peptide | sc-373896 P | 100 µg/0.5 ml | $69.00 |