SIRP-α/β Antibody (A-1): sc-17803

SIRP-α/β Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SIRP-α/β protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SIRP-α/β antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The SIRP family, which includes SIRP-α and SIRP-β, plays a crucial role in regulating immune responses and cellular signaling. SIRP-α, a transmembrane protein, is characterized by an extracellular domain containing three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. This structural configuration allows SIRP-α to interact with various signaling molecules, including the Src homology 2 domain-containing protein-tyrosine phosphatase SHP-2, thereby modulating the PI 3-kinase pathway. By negatively regulating this pathway, SIRP-α can influence cellular responses to growth factors and oncogenes, which is vital for maintaining cellular homeostasis and preventing uncontrolled cell proliferation. SIRP-β1, which shares extensive sequence homology with SIRP-α, lacks the cytoplasmic domain, indicating a divergence in function and regulatory mechanisms within the SIRP family. SIRP-γ, expressed predominantly on T cells and some B cells, further highlights the complexity of SIRP interactions, particularly through association with CD47, which signals unidirectionally. This intricate network of interactions underscores the importance of SIRP proteins in immune regulation and their potential as therapeutic targets in various diseases.

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SIRP-α/β Antibody (A-1) References:

1. Inhibition of EGFR-mediated phosphoinositide-3-OH kinase (PI3-K) signaling and glioblastoma phenotype by signal-regulatory proteins (SIRPs).  |  Wu, CJ., et al. 2000. Oncogene. 19: 3999-4010. PMID: 10962556
2. Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells.  |  Seiffert, M., et al. 2001. Blood. 97: 2741-9. PMID: 11313266
3. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.  |  Latour, S., et al. 2001. J Immunol. 167: 2547-54. PMID: 11509594
4. Positive regulation of phagocytosis by SIRPbeta and its signaling mechanism in macrophages.  |  Hayashi, A., et al. 2004. J Biol Chem. 279: 29450-60. PMID: 15123631
5. Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.  |  Brooke, G., et al. 2004. J Immunol. 173: 2562-70. PMID: 15294972
6. Transcriptional regulation of signal regulatory protein alpha1 inhibitory receptors by epidermal growth factor receptor signaling.  |  Kapoor, GS., et al. 2004. Cancer Res. 64: 6444-52. PMID: 15374953
7. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration.  |  Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
8. Surfactant protein D (Sp-D) binds to membrane-proximal domain (D3) of signal regulatory protein α (SIRPα), a site distant from binding domain of CD47, while also binding to analogous region on signal regulatory protein β (SIRPβ).  |  Fournier, B., et al. 2012. J Biol Chem. 287: 19386-98. PMID: 22511785
9. Identification of the major SHPTP2-binding protein that is tyrosine-phosphorylated in response to insulin.  |  Yamauchi, K., et al. 1995. J Biol Chem. 270: 17716-22. PMID: 7629070
10. A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.  |  Fujioka, Y., et al. 1996. Mol Cell Biol. 16: 6887-99. PMID: 8943344
11. A family of proteins that inhibit signalling through tyrosine kinase receptors.  |  Kharitonenkov, A., et al. 1997. Nature. 386: 181-6. PMID: 9062191
12. Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association.  |  Stofega, MR., et al. 1998. J Biol Chem. 273: 7112-7. PMID: 9507023

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRP-α/β Antibody (A-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRP-α/β Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRP-α/β Antibody (A-1) Specifications

Alternate Product Name: SIRP-α/β

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: SIRP-α/β

Epitope Species: human

Product Reactivity/Detection: SIRP-α and SIRP-β

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for SIRP-α/β Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SIRP-α antibody is SIRP-α Antibody (SE7C2): sc-23863.