Sil Antibody (A-6): sc-271910
- Sil Antibody (A-6) is a mouse monoclonal IgG2a κ Sil antibody provided at 200 µg/ml
- raised against amino acids 69-1262 mapping at the C-terminus of SIL of mouse origin
- Sil Antibody (A-6) is recommended for detection of Sil of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Sil Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Sil Antibody (A-6) for WB applications. These reagents are now offered in bundles with Sil Antibody (A-6) (see ordering information below).
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SEE ALSO...
Sil Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Sil protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sil (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sil protein, also known as SCL (stem cell leukemia), plays a crucial role in hematopoiesis and is essential for blood cell development. Sil (A-6) monoclonal antibody functions as a transcription factor that regulates gene expression involved in cell proliferation and differentiation, making Sil protein vital for normal blood cell development and function. Sil protein expression is tightly regulated throughout the cell cycle, peaking during mitosis and decreasing as cells transition to G1 phase. Dysregulation of Sil expression has been implicated in various hematological malignancies, including T-cell acute lymphoblastic leukemia, where TAL1 disruption leads to SIL-TAL1 fusion product formation. Overexpression of Sil in primary adenocarcinomas has been associated with increased metastatic potential, particularly in lung tumors characterized by heightened mitotic activity, highlighting Sil protein significance as a potential biomarker for cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sil Antibody (A-6) References:
- The SIL gene is required for mouse embryonic axial development and left-right specification. | Izraeli, S., et al. 1999. Nature. 399: 691-4. PMID: 10385121
- Analysis of the V(D)J recombination efficiency at lymphoid chromosomal translocation breakpoints. | Raghavan, SC., et al. 2001. J Biol Chem. 276: 29126-33. PMID: 11390401
- Measurement of SIL-TAL1 fusion gene transcripts associated with human T-cell lymphocytic leukemia by real-time reverse transcriptase-PCR. | Curry, JD. and Smith, MT. 2003. Leuk Res. 27: 575-82. PMID: 12681356
- Sil overexpression in lung cancer characterizes tumors with increased mitotic activity. | Erez, A., et al. 2004. Oncogene. 23: 5371-7. PMID: 15107824
- Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia. | Aplan, PD., et al. 1991. Mol Cell Biol. 11: 5462-9. PMID: 1922059
- Disruption of the human SCL locus by 'illegitimate' V-(D)-J recombinase activity. | Aplan, PD., et al. 1990. Science. 250: 1426-9. PMID: 2255914
- Cloning and characterization of a murine SIL gene. | Collazo-Garcia, N., et al. 1995. Genomics. 30: 506-13. PMID: 8825637
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sil Antibody (A-6) | sc-271910 | 200 µg/ml | $316.00 | |||
Sil Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-529284 | 200 µg Ab; 10 µg BP | $354.00 | |||
Sil Antibody (A-6): m-IgGκ BP-HRP Bundle | sc-522097 | 200 µg Ab, 40 µg BP | $354.00 | |||
Sil Antibody (A-6): m-IgG2a BP-HRP Bundle | sc-547292 | 200 µg Ab; 10 µg BP | $354.00 | |||
Sil Antibody (A-6) AC | sc-271910 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Sil Antibody (A-6) HRP | sc-271910 HRP | 200 µg/ml | $316.00 | |||
Sil Antibody (A-6) FITC | sc-271910 FITC | 200 µg/ml | $330.00 | |||
Sil Antibody (A-6) PE | sc-271910 PE | 200 µg/ml | $343.00 | |||
Sil Antibody (A-6) Alexa Fluor® 488 | sc-271910 AF488 | 200 µg/ml | $357.00 | |||
Sil Antibody (A-6) Alexa Fluor® 546 | sc-271910 AF546 | 200 µg/ml | $357.00 | |||
Sil Antibody (A-6) Alexa Fluor® 594 | sc-271910 AF594 | 200 µg/ml | $357.00 | |||
Sil Antibody (A-6) Alexa Fluor® 647 | sc-271910 AF647 | 200 µg/ml | $357.00 | |||
Sil Antibody (A-6) Alexa Fluor® 680 | sc-271910 AF680 | 200 µg/ml | $357.00 | |||
Sil Antibody (A-6) Alexa Fluor® 790 | sc-271910 AF790 | 200 µg/ml | $357.00 |