Siglec-5/14 Antibody (E-1): sc-374585
- Siglec-5/14 Antibody (E-1) is a mouse monoclonal IgG3 κ Siglec-5/14 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 135-173 within an extracellular domain of Siglec-5 of human origin
- recommended for detection of Siglec-5 and Siglec-14 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Siglec-5/14 Antibody (E-1) for WB applications. These reagents are now offered in bundles with Siglec-5/14 Antibody (E-1) (see ordering information below).
Siglec-5/14 Antibody (E-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects Siglec-5/14 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Siglec-5/14 antibody (E-1) is available as non-conjugated. Siglec-5 plays a crucial role in immune regulation, particularly in recognizing sialylated ligands, which are important for cell-cell interactions and immune responses. Siglec-5 binds both alpha2,3-linked and alpha2,6-linked sialic acid, facilitating its function in modulating immune cell activity. Siglec-5 expression on monocytes and neutrophils suggests involvement in immune response, while its absence in certain leukemic cell lines indicates a potential role in diagnosing and monitoring acute myeloid leukemia (AML). Siglec-14, predominantly expressed in hematopoietic tissues, lung, and testis, further highlights these proteins′ significance in various biological contexts. Diverse isoforms of Siglec-5, characterized by different extracellular domains and cytoplasmic tail lengths, underscore the complexity of regulatory functions in the immune system.
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Siglec-5/14 Antibody (E-1) References:
- Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions. | Connolly, NP., et al. 2002. Br J Haematol. 119: 221-38. PMID: 12358929
- Characterization of Siglec-5 (CD170) expression and functional activity of anti-Siglec-5 antibodies on human phagocytes. | Erickson-Miller, CL., et al. 2003. Exp Hematol. 31: 382-8. PMID: 12763136
- Identification of the CD33-related Siglec receptor, Siglec-5 (CD170), as a useful marker in both normal myelopoiesis and acute myeloid leukaemias. | Virgo, P., et al. 2003. Br J Haematol. 123: 420-30. PMID: 14617000
- Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. | Avril, T., et al. 2005. J Biol Chem. 280: 19843-51. PMID: 15769739
- Sialoside-binding macrophage lectins in phagocytosis of apoptotic bodies. | Rapoport, EM., et al. 2005. Biochemistry (Mosc). 70: 330-8. PMID: 15823088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Siglec-5/14 Antibody (E-1) | sc-374585 | 200 µg/ml | $316.00 | |||
Siglec-5/14 Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-529776 | 200 µg Ab; 10 µg BP | $354.00 | |||
Siglec-5/14 Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-522785 | 200 µg Ab, 40 µg BP | $354.00 | |||
Siglec-5/14 (E-1) Neutralizing Peptide | sc-374585 P | 100 µg/0.5 ml | $68.00 |