SIGIRR Antibody (A-4): sc-271864

SIGIRR Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SIGIRR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SIGIRR antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIGIRR protein, also known as single immunoglobulin domain-containing IL-1 receptor-related protein or Toll/interleukin-1 receptor 8 (TIR8), plays a crucial role as a negative regulator of the interleukin-1 receptor and Toll-like receptor signaling pathways, which are essential for the immune response. By inhibiting these pathways, SIGIRR helps to modulate inflammation and prevent excessive immune reactions, thereby conferring resistance to infections such as those caused by Pseudomonas aeruginosa in corneal tissues. SIGIRR can form complexes with key signaling proteins, including IL-1 receptor 1, MYD88, IRAK1, and TRAF6, upon stimulation with interleukin-1 or lipopolysaccharides, highlighting its importance in immune signaling. This single-pass type III membrane protein is predominantly expressed in the kidney, lung, and gut, underscoring its significance in maintaining immune homeostasis in these vital organs.

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SIGIRR Antibody (A-4) References:

1. Identification and characterization of SIGIRR, a molecule representing a novel subtype of the IL-1R superfamily.  |  Thomassen, E., et al. 1999. Cytokine. 11: 389-99. PMID: 10346978
2. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling.  |  Wald, D., et al. 2003. Nat Immunol. 4: 920-7. PMID: 12925853
3. Extracellular and intracellular decoys in the tuning of inflammatory cytokines and Toll-like receptors: the new entry TIR8/SIGIRR.  |  Mantovani, A., et al. 2004. J Leukoc Biol. 75: 738-42. PMID: 14673019
4. Unique pattern of expression and inhibition of IL-1 signaling by the IL-1 receptor family member TIR8/SIGIRR.  |  Polentarutti, N., et al. 2003. Eur Cytokine Netw. 14: 211-8. PMID: 14715412
5. SIGIRR inhibits interleukin-1 receptor- and toll-like receptor 4-mediated signaling through different mechanisms.  |  Qin, J., et al. 2005. J Biol Chem. 280: 25233-41. PMID: 15866876
6. SIGIRR promotes resistance against Pseudomonas aeruginosa keratitis by down-regulating type-1 immunity and IL-1R1 and TLR4 signaling.  |  Huang, X., et al. 2006. J Immunol. 177: 548-56. PMID: 16785552
7. Up-regulation of MyD88s and SIGIRR, molecules inhibiting Toll-like receptor signaling, in monocytes from septic patients.  |  Adib-Conquy, M., et al. 2006. Crit Care Med. 34: 2377-85. PMID: 16850005
8. SIGIRR Negatively Regulates IL-36-Driven Psoriasiform Inflammation and Neutrophil Infiltration in the Skin.  |  Giannoudaki, E., et al. 2021. J Immunol. 207: 651-660. PMID: 34253575
9. SIGIRR Mutation in Human Necrotizing Enterocolitis (NEC) Disrupts STAT3-Dependent microRNA Expression in Neonatal Gut.  |  Yu, W., et al. 2022. Cell Mol Gastroenterol Hepatol. 13: 425-440. PMID: 34563711
10. A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells.  |  Ueno-Shuto, K., et al. 2022. Int J Mol Sci. 23: PMID: 35887095
11. Dominant-Negative Form of SIGIRR: SIGIRRΔE8 Promotes Tumor Growth Through Regulation of Metabolic Pathways.  |  Bodaszewska-Lubas, M., et al. 2022. J Interferon Cytokine Res. 42: 482-492. PMID: 35900274
12. SIGIRR deficiency contributes to CD4 T cell abnormalities by facilitating the IL1/C/EBPβ/TNF-α signaling axis in rheumatoid arthritis.  |  Teng, X., et al. 2022. Mol Med. 28: 135. PMID: 36401167

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIGIRR Antibody (A-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-SIGIRR Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIGIRR Antibody (A-4) Specifications

Alternate Product Name: SIGIRR

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 377-394

Epitope Target: SIGIRR

Epitope Species: human

Product Reactivity/Detection: SIGIRR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for SIGIRR Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin