SHP Antibody (H-5): sc-271511

SHP Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects SHP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-SHP antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SHP, also known as small heterodimer partner, is an orphan nuclear receptor that plays a crucial role in regulating metabolic processes by modulating the activity of other nuclear receptors. SHP lacks a DNA binding domain, which distinguishes SHP from other nuclear receptors, allowing SHP to function primarily as a negative regulator. SHP is predominantly expressed in the liver, fetal liver, adrenal gland, spleen, and small intestine, highlighting SHP′s importance in metabolic regulation and homeostasis. SHP interacts with various nuclear receptors, including thyroid receptors, retinoic acid receptors, and estrogen receptors, inhibiting their activity through mechanisms such as dimerization and competition for coactivators. SHP is involved in the transcriptional regulation of NF-κB in macrophages, suggesting SHP′s role as a modulator in inflammatory responses. SHP regulation is critical for maintaining bile acid homeostasis, as SHP is induced by bile acids through the farnesoid X receptor (FXR), providing a protective mechanism against bile acid-induced hepatocyte damage in cholestatic conditions.

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SHP Antibody (H-5) References:

1. The orphan nuclear receptor SHP utilizes conserved LXXLL-related motifs for interactions with ligand-activated estrogen receptors.  |  Johansson, L., et al. 2000. Mol Cell Biol. 20: 1124-33. PMID: 10648597
2. Short heterodimer partner (SHP) orphan nuclear receptor inhibits the transcriptional activity of aryl hydrocarbon receptor (AHR)/AHR nuclear translocator (ARNT).  |  Klinge, CM., et al. 2001. Arch Biochem Biophys. 390: 64-70. PMID: 11368516
3. The orphan nuclear receptor, shp, mediates bile acid-induced inhibition of the rat bile acid transporter, ntcp.  |  Denson, LA., et al. 2001. Gastroenterology. 121: 140-7. PMID: 11438503
4. The orphan nuclear receptor small heterodimer partner as a novel coregulator of nuclear factor-kappa b in oxidized low density lipoprotein-treated macrophage cell line RAW 264.7.  |  Kim, YS., et al. 2001. J Biol Chem. 276: 33736-40. PMID: 11448950
5. The agonist activity of tamoxifen is inhibited by the short heterodimer partner orphan nuclear receptor in human endometrial cancer cells.  |  Klinge, CM., et al. 2002. Endocrinology. 143: 853-67. PMID: 11861507
6. Inhibition of aromatase transcription via promoter II by short heterodimer partner in human preadipocytes.  |  Kovacic, A., et al. 2004. Mol Endocrinol. 18: 252-9. PMID: 14593077
7. Overexpression of short heterodimer partner recovers impaired glucose-stimulated insulin secretion of pancreatic beta-cells overexpressing UCP2.  |  Suh, YH., et al. 2004. J Endocrinol. 183: 133-44. PMID: 15525581
8. Role of nuclear receptor SHP in metabolism and cancer.  |  Zhang, Y., et al. 2011. Biochim Biophys Acta. 1812: 893-908. PMID: 20970497
9. SHP-1 Regulates CD8+ T Cell Effector Function but Plays a Subtle Role with SHP-2 in T Cell Exhaustion Due to a Stage-Specific Nonredundant Functional Relay.  |  Hou, B., et al. 2024. J Immunol. 212: 397-409. PMID: 38088801
10. An orphan nuclear hormone receptor that lacks a DNA binding domain and heterodimerizes with other receptors.  |  Seol, W., et al. 1996. Science. 272: 1336-9. PMID: 8650544
11. Structure and expression of the orphan nuclear receptor SHP gene.  |  Lee, HK., et al. 1998. J Biol Chem. 273: 14398-402. PMID: 9603951
12. Inhibition of estrogen receptor action by the orphan receptor SHP (short heterodimer partner).  |  Seol, W., et al. 1998. Mol Endocrinol. 12: 1551-7. PMID: 9773978

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SHP Antibody (H-5) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-SHP Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SHP Antibody (H-5) Specifications

Alternate Product Name: NR0B2

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-160

Epitope Target: SHP

Epitope Species: human

Product Reactivity/Detection: SHP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for SHP Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin