Shigatoxin Antibody (STX-1): sc-69865
- Shigatoxin Antibody (STX-1) is a mouse monoclonal IgG1 κ Shigatoxin antibody provided at 200 µg/ml
- raised against Shigatoxin of E. coli origin
- Shigatoxin Antibody (STX-1) is recommended for detection of Shigatoxin by WB
- Anti-Shigatoxin Antibody (STX-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Shigatoxin Antibody (STX-1) for WB applications. These reagents are now offered in bundles with Shigatoxin Antibody (STX-1) (see ordering information below).
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Shigatoxin Antibody (STX-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Shigatoxin protein by western blotting (WB). Anti-Shigatoxin antibody (STX-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Shigatoxin 1 (Stx1), the target of Shigatoxin monoclonal antibody (STX-1), is a critical virulence factor produced by Shigella dysenteriae type 1 and enterohemorrhagic Escherichia coli, and plays a significant role in the pathogenesis of hemolytic uremic syndrome (HUS), which is the leading cause of acute renal failure in children globally. Stx1′s function is particularly important as Stx1 disrupts protein synthesis in glomerular epithelial cells by increasing arachidonate release and eicosanoid production, leading to a cascade of inflammatory responses. Stx1 also enhances the release of pro-inflammatory cytokines from renal epithelial cells, contributing to the clinical manifestations of HUS, such as microangiopathic hemolytic anemia and low platelet counts. Furthermore, Stx1′s interaction with globotriaosylceramide is linked to brain injury in HUS, highlighting Stx1′s critical role in both renal and neurological complications. Understanding the mechanisms of Stx1′s action is essential for developing therapeutic strategies to mitigate these effects and improve patient outcomes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Shigatoxin Antibody (STX-1) References:
- Shiga toxin-1 regulation of cytokine production by human glomerular epithelial cells. | Hughes, AK., et al. 2001. Nephron. 88: 14-23. PMID: 11340345
- Molecular basis for high renal cell sensitivity to the cytotoxic effects of shigatoxin-1: upregulation of globotriaosylceramide expression. | Hughes, AK., et al. 2002. J Am Soc Nephrol. 13: 2239-45. PMID: 12191968
- Shigatoxin-1 binding and receptor expression in human kidneys do not change with age. | Ergonul, Z., et al. 2003. Pediatr Nephrol. 18: 246-53. PMID: 12644917
- Escherichia coli Shiga toxin 1 and TNF-alpha induce cytokine release by human cerebral microvascular endothelial cells. | Eisenhauer, PB., et al. 2004. Microb Pathog. 36: 189-96. PMID: 15001224
- Regulation of proinflammatory cytokine expression by Shiga toxin 1 and/or lipopolysaccharides in the human monocytic cell line THP-1. | Harrison, LM., et al. 2004. Infect Immun. 72: 2618-27. PMID: 15102770
- Inhibition of p38 mitogen-activated protein kinase ameliorates cytokine up-regulated shigatoxin-1 toxicity in human brain microvascular endothelial cells. | Stricklett, PK., et al. 2005. J Infect Dis. 191: 461-71. PMID: 15633106
- Shiga toxin-1 regulation of cytokine production by human proximal tubule cells. | Hughes, AK., et al. 1998. Kidney Int. 54: 1093-106. PMID: 9767525
- Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. | Strockbine, NA., et al. 1985. Infect Immun. 50: 695-700. PMID: 3905611
- Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells. | Hughes, AK., et al. 2002. BMC Nephrol. 3: 6. PMID: 12181081
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shigatoxin Antibody (STX-1) | sc-69865 | 200 µg/ml | $322.00 | |||
Shigatoxin Antibody (STX-1): m-IgG Fc BP-HRP Bundle | sc-526992 | 200 µg Ab; 10 µg BP | $361.00 | |||
Shigatoxin Antibody (STX-1): m-IgG1 BP-HRP Bundle | sc-532365 | 200 µg Ab; 20 µg BP | $361.00 | |||
Shigatoxin Antibody (STX-1) AC | sc-69865 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Shigatoxin Antibody (STX-1) HRP | sc-69865 HRP | 200 µg/ml | $322.00 | |||
Shigatoxin Antibody (STX-1) FITC | sc-69865 FITC | 200 µg/ml | $336.00 | |||
Shigatoxin Antibody (STX-1) PE | sc-69865 PE | 200 µg/ml | $349.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 488 | sc-69865 AF488 | 200 µg/ml | $364.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 546 | sc-69865 AF546 | 200 µg/ml | $364.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 594 | sc-69865 AF594 | 200 µg/ml | $364.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 647 | sc-69865 AF647 | 200 µg/ml | $364.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 680 | sc-69865 AF680 | 200 µg/ml | $364.00 | |||
Shigatoxin Antibody (STX-1) Alexa Fluor® 790 | sc-69865 AF790 | 200 µg/ml | $364.00 |