Shc Antibody (PG-797): sc-967

Shc Antibody (PG-797) is a mouse monoclonal IgG1 kappa light chain antibody that detects Shc in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Shc monoclonal antibody (PG-797) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Shc plays a crucial role in cellular signaling pathways, particularly in the context of growth factor receptor activation. Shc acts as an adapter protein that links activated receptor tyrosine kinases to downstream signaling molecules, facilitating the transmission of signals that lead to cellular responses such as proliferation and differentiation. Shc is known to interact with several key proteins, including GRB2 and Sos, which are essential for the activation of the Ras signaling pathway, a critical pathway involved in cell growth and survival. The ability of Shc to form complexes with these proteins underscores its importance in mediating insulin signaling and other mitogenic responses, highlighting its role as a pivotal player in the intricate network of cellular communication.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Shc Antibody (PG-797) References:

1. Shc proteins are phosphorylated and regulated by the v-Src and v-Fps protein-tyrosine kinases.  |  McGlade, J., et al. 1992. Proc Natl Acad Sci U S A. 89: 8869-73. PMID: 1409579
2. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.  |  Pelicci, G., et al. 1992. Cell. 70: 93-104. PMID: 1623525
3. Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases.  |  Ellis, C., et al. 1990. Nature. 343: 377-81. PMID: 1689011
4. Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.  |  Morrison, DK., et al. 1990. Mol Cell Biol. 10: 2359-66. PMID: 1691440
5. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
6. Oncogenes and signal transduction.  |  Cantley, LC., et al. 1991. Cell. 64: 281-302. PMID: 1846320
7. Signal transduction by receptors with tyrosine kinase activity.  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
8. Shc and the mechanotransduction of cellular anchorage and metastasis.  |  Terada, LS. 2019. Small GTPases. 10: 64-71. PMID: 28632027
9. The neuronal Shc adaptor in Alzheimer's Disease.  |  Triaca, V., et al. 2018. Aging (Albany NY). 10: 5-6. PMID: 29362290
10. Shc Is Implicated in Calreticulin-Mediated Sterile Inflammation in Alcoholic Hepatitis.  |  Li, Y., et al. 2023. Cell Mol Gastroenterol Hepatol. 15: 197-211. PMID: 36122677
11. The p66Shc Redox Protein and the Emerging Complications of Diabetes.  |  Biondi, G., et al. 2023. Int J Mol Sci. 25: PMID: 38203279
12. Interaction of Shc with the zeta chain of the T cell receptor upon T cell activation.  |  Ravichandran, KS., et al. 1993. Science. 262: 902-5. PMID: 8235613

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Shc Antibody (PG-797) has 83 citations in a variety of scientific publications.

Primary antibodies like Anti-Shc Antibody (PG-797) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Shc Antibody (PG-797) Specifications

Alternate Product Name: Shc

Product Clone Name: PG-797

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 476-583

Epitope Target: Shc

Epitope Species: human

Product Reactivity/Detection: Shc p66, p52 and p46

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 83 citations in various scientific publications

Available Conjugates/Forms for Shc Antibody (PG-797): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin