SH3D19 Antibody (G-6): sc-377282
- SH3D19 Antibody (G-6) is a mouse monoclonal IgG1 κ SH3D19 antibody provided at 200 µg/ml
- raised against amino acids 441-740 mapping near the C-terminus of SH3D19 of human origin
- SH3D19 Antibody (G-6) is recommended for detection of SH3D19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SH3D19 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SH3D19 Antibody (G-6) for WB and IHC(P) applications. These reagents are now offered in bundles with SH3D19 Antibody (G-6) (see ordering information below).
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SH3D19 Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects SH3D19 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SH3D19 antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SH3D19, also known as SH3 domain-containing protein 19, EBP, EEN-binding protein, EVE1, or Kryn, is a 790 amino acid protein widely expressed, with highest levels found in kidney, heart, lung, liver, skeletal muscle, and small intestine. SH3D19 primarily localizes in cytoplasm but can be recruited to nucleus by a fusion protein known as MLL-EEN (mixed-lineage leukemia-endophilin), highlighting potential role in cellular signaling pathways. SH3D19 regulates ADAM (A disintegrin and metalloproteases) proteins in epidermal growth factor receptor-ligand shedding pathways, crucial for various physiological processes, including cell proliferation and differentiation. SH3D19 contains five SH3 domains, important for protein-protein interactions, and may suppress Ras-induced cellular transformation and activation of downstream signaling proteins such as Elk-1. Translocation events involving SH3D19 have been implicated in acute myeloid leukemia, suggesting a significant role in carcinogenesis. Alternative splicing of SH3D19 results in expression of five distinct isoforms, adding complexity to functional repertoire in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SH3D19 Antibody (G-6) References:
- Gene expression of Sh3d19, a novel adaptor protein with five Src homology 3 domains, in anagen mouse hair follicles. | Shimomura, Y., et al. 2003. J Dermatol Sci. 31: 43-51. PMID: 12615363
- Identification and characterization of EBP, a novel EEN binding protein that inhibits Ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein. | Yam, JW., et al. 2004. Blood. 103: 1445-53. PMID: 14551139
- ADAM binding protein Eve-1 is required for ectodomain shedding of epidermal growth factor receptor ligands. | Tanaka, M., et al. 2004. J Biol Chem. 279: 41950-9. PMID: 15280379
- Identification of novel Runx1 (AML1) translocation partner genes SH3D19, YTHDf2, and ZNF687 in acute myeloid leukemia. | Nguyen, TT., et al. 2006. Genes Chromosomes Cancer. 45: 918-32. PMID: 16858696
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH3D19 Antibody (G-6) | sc-377282 | 200 µg/ml | $316.00 | |||
SH3D19 Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-530000 | 200 µg Ab; 10 µg BP | $354.00 | |||
SH3D19 Antibody (G-6): m-IgGκ BP-HRP Bundle | sc-523264 | 200 µg Ab, 40 µg BP | $354.00 | |||
SH3D19 Antibody (G-6): m-IgG1 BP-HRP Bundle | sc-543812 | 200 µg Ab; 20 µg BP | $354.00 | |||
SH3D19 Antibody (G-6) AC | sc-377282 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SH3D19 Antibody (G-6) HRP | sc-377282 HRP | 200 µg/ml | $316.00 | |||
SH3D19 Antibody (G-6) FITC | sc-377282 FITC | 200 µg/ml | $330.00 | |||
SH3D19 Antibody (G-6) PE | sc-377282 PE | 200 µg/ml | $343.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 488 | sc-377282 AF488 | 200 µg/ml | $357.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 546 | sc-377282 AF546 | 200 µg/ml | $357.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 594 | sc-377282 AF594 | 200 µg/ml | $357.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 647 | sc-377282 AF647 | 200 µg/ml | $357.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 680 | sc-377282 AF680 | 200 µg/ml | $357.00 | |||
SH3D19 Antibody (G-6) Alexa Fluor® 790 | sc-377282 AF790 | 200 µg/ml | $357.00 |