Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SH3BGRL CRISPR/Cas9 KO Plasmid (h): sc-406958
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SH3BGRL CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SH3BGRL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SH3BGRL Antibody (E-5): sc-377108
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH3BGRL CRISPR/Cas9 KO Plasmid (h) | sc-406958 | 20 µg | $397.00 |
Overview
SH3BGRL (SH3 domain binding glutamate rich protein like) encodes a small adaptor-like protein that can modulate protein–protein interactions involving SH3-domain signaling complexes. It has been linked to regulation of cytoskeletal dynamics, redox-responsive signaling, and stress-associated pathways that influence cell growth and survival decisions. Altered SH3BGRL expression has been reported across multiple tumor contexts and has been studied in relation to invasive behavior and changes in proliferative signaling. These features make SH3BGRL relevant for mechanistic work on signal transduction, cell-state regulation, and pathway rewiring in disease models.
SH3BGRL CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SH3BGRL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SH3BGRL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SH3BGRL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SH3BGRL protein expression.
This CRISPR knockout system enables efficient generation of SH3BGRL-deficient cell models for investigation of SH3BGRL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SH3BGRL exon(s) critical for SH3BGRL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SH3BGRL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SH3BGRL CRISPR/Cas9 KO Plasmid (h) and SH3BGRL CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SH3BGRL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SH3BGRL HDR Plasmid (h) and SH3BGRL HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SH3BGRL homology arms to support homology-directed repair at defined SH3BGRL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.