Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SH2D1A CRISPR/Cas9 KO Plasmid (m): sc-422910
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SH2D1A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SH2D1A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SH2D1A Antibody (A-8): sc-398118
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH2D1A CRISPR/Cas9 KO Plasmid (m) | sc-422910 | 20 µg | $397.00 |
Overview
Sh2d1a encodes SH2D1A (SAP), an SH2-domain adaptor predominantly expressed in T cells and NK cells that couples SLAM family receptors to downstream signaling. By coordinating receptor-proximal complexes and modulating kinases and phosphatases, SH2D1A shapes immunological synapse formation, cytotoxic effector functions, and T cell help during antiviral and humoral immune responses. Disruption of SH2D1A-dependent signaling perturbs lymphocyte activation and immune homeostasis, making it relevant to studies of immune dysregulation, susceptibility to viral infection, and lymphoproliferative phenotypes. Mouse Sh2d1a models are widely used to interrogate SLAM–SAP axis contributions to germinal center biology and NK/T cell-mediated control of infected or transformed cells.
SH2D1A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sh2d1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sh2d1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sh2d1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SH2D1A protein expression.
This CRISPR knockout system enables efficient generation of Sh2d1a-deficient cell models for investigation of SH2D1A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sh2d1a exon(s) critical for SH2D1A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sh2d1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SH2D1A CRISPR/Cas9 KO Plasmid (m) and SH2D1A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sh2d1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SH2D1A HDR Plasmid (m) and SH2D1A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sh2d1a homology arms to support homology-directed repair at defined Sh2d1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.